The RNA sequencing data sets available for different fish species show a potentially high variety of forms of enzymes just in teleosts. This is primarily considered an effect of the first round of whole-genome duplication with mutations in duplicated genes (isozymes) and alternative splicing of mRNA (isoforms). However, the abundance of the mRNA transcript variants is not necessarily reflected in the abundance of active forms of proteins. We have investigated the transcriptional profiles of two enzymes, aralkylamine N-acetyltransferase (AANAT: EC 2.3.1.87) and N-acetylserotonin O-methyltransferase (ASMT: EC 2.1.1.4), in the eyeball, brain, intestines, spleen, heart, liver, head kidney, gonads, and skin of the European flounder (Platichthys flesus). High-throughput next-generation sequencing technology NovaSeq6000 was used to generate 500M sequencing reads. These were then assembled and filtered producing 75k reliable contigs. Gene ontology (GO) terms were assigned to the majority of annotated contigs/unigenes based on the results of PFAM, PANTHER, UniProt, and InterPro protein database searches. BUSCOs statistics for metazoa, vertebrata, and actinopterygii databases showed that the reported transcriptome represents a high level of completeness. In this article, we show how to preselect transcripts encoding the active enzymes (isozymes or isoforms), using AANAT and ASMT in the European flounder as the examples. The data can be used as a tool to design the experiments as well as a basis for discussion of diversity of enzyme forms and their physiological relevance in teleosts.

可用于不同鱼类的RNA测序数据集显示出硬骨鱼类中存在多种潜在形式的酶。这主要被认为是第一轮全基因组复制对重复基因（同功酶）中的突变和mRNA的可变剪接（同工型）产生的影响。但是，mRNA转录变体的丰度并不一定反映在蛋白质活性形式的丰度中。我们研究了眼球，大脑，肠，脾脏，心脏，肝，头肾，性腺和欧洲比目鱼的皮肤（斜纹肌）。高通量下一代测序技术NovaSeq6000用于生成500M测序读数。然后将它们组装并过滤，产生75,000个可靠重叠群。根据PFAM，PANTHER，UniProt和InterPro蛋白质数据库搜索的结果，将基因本体论（GO）术语分配给了大多数带注释的重叠群/单基因。BUSCO对后生动物，椎骨和放线菌数据库的统计表明，报告的转录组代表高度的完整性。在本文中，我们以欧洲比目鱼为例，展示了如何预先选择编码活性酶（同工酶或同工型）的转录本。该数据可以用作设计实验的工具，也可以作为讨论硬骨粉中酶形式多样性及其生理相关性的基础。