Aims

The pea seeds (Pisum sativum) hosts a complex microbial community that is exposed to environmental constraints modulated by biotic, abiotic and anthropogenic factors. The aim of this study was to explore the effect of geographical origins on the composition of the microbial communities associated with pea seeds from storage locations in different countries. These seeds are used by Roquette Frères® as source proteins for human food after industrial extraction process of their proteins.

Methods

The high-throughput sequencing of two phylogenetic markers (V3-V4 regions of the 16S rRNA gene and ITS2 region) was used to evaluate the structure of the microbial community associated with pea seeds. The pea seeds were harvested by different suppliers from three countries (France, Sweden and Canada) with different climates and stored in six cooperatives. Representative microbial strains of dominant species were isolated and characterized using phenotypic methods.

Results

Although the pea seed microbiota shared a dominant core microbiota at the genus level, seeds showed a species-specific, highly unique microbial signature with variable abundances according to origin. Origin was identified as the main driver of the composition of the pea seed microbiota.

Conclusions

These results provide an initial insight into the broad factors such as country and cooperative that may determine the assembly of the pea microbiota before processing.

中文翻译：

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与不同地理起源的豌豆种子（Pisum sativum）相关的微生物群落

目的

豌豆种子（Pisum sativum）拥有一个复杂的微生物群落，受到生物，非生物和人为因素调节的环境限制。这项研究的目的是探讨地理起源对来自不同国家存储地点的豌豆种子相关微生物群落组成的影响。RoquetteFrères®使用这些种子作为蛋白质的工业提取工艺之后，可作为人类食品的源蛋白。

方法

两个系统发育标记（16S rRNA基因的V3-V4区和ITS2区）的高通量测序用于评估与豌豆种子相关的微生物群落的结构。不同气候的三个国家（法国，瑞典和加拿大）的不同供应商收获了豌豆种子，并存储在六个合作社中。使用表型方法分离并表征了优势种的代表性微生物菌株。

结果

尽管豌豆种子微生物群在属水平上共有一个占主导地位的核心微生物群，但是种子显示出一种物种特异性的，高度独特的微生物特征，并且根据来源具有不同的丰度。起源被确定为豌豆种子微生物群组成的主要驱动力。

结论

这些结果提供了对诸如乡村和合作社等广泛因素的初步了解，这些因素可能决定加工前豌豆菌群的组装。