The roe deer is a monoestrous species with a very short rutting season. The present work reports the most suitable period for collecting epididymal sperm and describes the effect of two cooling rates on the post-thaw quality of sperm. Testes were collected 24–48 h after death. Samples of sperm flushed from the epididymis were subjected to either (1) dilution in a Tris-citric acid-glucose-egg yolk-based medium with glycerol, and slow freezing in straws, or (2) dilution in the same extender but replacing the glycerol with 100 mM of sucrose, and ultra-rapid freezing in pellets. Sperm motility, acrosome and membrane integrity, morphometry and morphological abnormalities were analysed before and after cryopreservation. Spermatogenic activity was investigated via histological examination of testis sections. Several testes collected between April, May and September showed no spermatogenic activity. All those collected in June–August showed spermatogenic activity. No significant difference was detected in the cryoresistance ratios associated with the conventional slow freezing, between sperm collected during the pre-rutting (April–May) and rutting (June–August) periods. No significant differences were seen between the slow-frozen-thawed and the ultra-rapid-frozen-thawed sperm in terms of percentage of viable sperm or the percentage of sperm with morphological abnormalities. Slow freezing returned significantly better (P<0.05) values for post-thaw acrosome integrity (43.3% vs. 25.0%) and straight-line velocity (19 μm/s vs. 4 μm/s). For both freezing methods, sperm heads were smaller post-thawing than pre-freezing (P<0.001). In conclusion, both the pre-rutting and rutting season are suitable periods for freezing roe deer sperm. Ultra-rapid freezing did not provide suitable results.

er是一种单发种，发情季节很短。本工作报告了收集附睾精子的最合适时期，并描述了两种冷却速率对解冻后精子质量的影响。死后24-48小时收集睾丸。从附睾中冲洗出的精子样品在（1）用甘油的Tris-柠檬酸-葡萄糖-蛋黄基培养基中进行稀释，并在吸管中缓慢冷冻，或（2）在相同的稀释剂中进行稀释，但更换甘油和100 mM蔗糖，并在颗粒中超快速冷冻。冷冻前后对精子活力，顶体和膜完整性，形态和形态异常进行了分析。通过睾丸组织切片检查生精活性。在四月之间收集了几份睾丸，五月和九月显示没有生精活性。在六月至八月收集的所有那些都显示出生精活性。在常规发情期（4月至5月）和发情期（6月至8月）之间收集的精子之间，与常规慢速冷冻相关的抗冻比没有发现显着差异。慢冻融精子与超速冻融精子在活精子百分数或形态异常精子百分数上均无显着差异。慢速冷冻效果明显更好（在发情前（4月至5月）和发情（6月至8月）期间收集的精子之间。慢冻融精子与超速冻融精子在活精子百分数或形态异常精子百分数上均无显着差异。慢速冷冻效果明显更好（在发情前（4月至5月）和发情（6月至8月）之间收集的精子之间。慢冻融精子和超速冻融精子在活精子百分数或形态异常精子百分数上均无显着差异。慢速冷冻效果明显更好（解冻后顶体完整性（43.3％对25.0％）和直线速度（19μm/ s对4μm/ s）的P <0.05）值。对于这两种冷冻方法，解冻后的精子头均比冷冻前小（P <0.001）。总之，前发情和发情季节都是冷冻ro精子的合适时期。超快速冷冻未提供合适的结果。