The structural protein VP3 of infectious bursal disease virus (IBDV) plays a critical role in viral assembly, replication, immune escape, and anti-apoptosis. Interaction between VP3 and host protein factors can affect stages in the viral replication cycle. In this study, 137 host proteins interacting with VP3 protein were screened through liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics approach. The functions and relevance of the proteins were obtained through bioinformatics analysis. Most VP3-interacting proteins were linked to binding, catalytic activity, and structural molecular activity, and performed functions in cell parts and cells. Biological functions of VP3-interacting proteins were mainly relevant to "Cytoskeleton", "Translation", and "Signal transduction mechanisms", involving ribosomes, "Tight junction", regulation of actin cytoskeleton, and other pathways. Six potential VP3-interacting proteins in host cells were knocked down, and vimentin, myosin-9, and annexin A2 were found to be related to IBDV replication. This study would help explore regulatory pathways and cellular mechanisms in IBDV-infected cells, and also provided clues for the in-depth study of VP3 biological functions and IBDV replication or pathogenesis.

传染性法氏囊病病毒 (IBDV) 的结构蛋白 VP3 在病毒组装、复制、免疫逃逸和抗凋亡中起关键作用。VP3 和宿主蛋白因子之间的相互作用会影响病毒复制周期的各个阶段。在这项研究中，通过基于液相色谱-串联质谱 (LC-MS/MS) 的蛋白质组学方法筛选了 137 种与 VP3 蛋白相互作用的宿主蛋白。通过生物信息学分析获得蛋白质的功能和相关性。大多数 VP3 相互作用蛋白与结合、催化活性和结构分子活性有关，并在细胞部分和细胞中发挥作用。VP3相互作用蛋白的生物学功能主要与“细胞骨架”、“翻译”和“信号转导机制”有关，涉及核糖体、“紧密连接”、肌动蛋白细胞骨架的调节和其他途径。宿主细胞中六种潜在的 VP3 相互作用蛋白被击倒，波形蛋白、肌球蛋白 9 和膜联蛋白 A2 被发现与 IBDV 复制有关。本研究将有助于探索IBDV感染细胞的调控途径和细胞机制，也为深入研究VP3生物学功能和IBDV复制或发病机制提供线索。