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Mass spectrometry-based proteomic analysis of potential infectious bursal disease virus VP3-interacting proteins in chicken embryo fibroblasts cells

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Abstract

The structural protein VP3 of infectious bursal disease virus (IBDV) plays a critical role in viral assembly, replication, immune escape, and anti-apoptosis. Interaction between VP3 and host protein factors can affect stages in the viral replication cycle. In this study, 137 host proteins interacting with VP3 protein were screened through liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics approach. The functions and relevance of the proteins were obtained through bioinformatics analysis. Most VP3-interacting proteins were linked to binding, catalytic activity, and structural molecular activity, and performed functions in cell parts and cells. Biological functions of VP3-interacting proteins were mainly relevant to "Cytoskeleton", "Translation", and "Signal transduction mechanisms", involving ribosomes, "Tight junction", regulation of actin cytoskeleton, and other pathways. Six potential VP3-interacting proteins in host cells were knocked down, and vimentin, myosin-9, and annexin A2 were found to be related to IBDV replication. This study would help explore regulatory pathways and cellular mechanisms in IBDV-infected cells, and also provided clues for the in-depth study of VP3 biological functions and IBDV replication or pathogenesis.

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Acknowledgements

The present studies were sequenced by Guangzhou Gene Denovo Biotechnology Co., Ltd.

Funding

This work was supported by National Key R&D Program of China (Grant No. 2018YFD0500106), Jiangsu Provincial Natural Science Foundation of China (Grant No. BK20180299), Jiangsu Agriculture Science and Technology Innovation Fund (CX(19)3019, CX(20)3095), and National Natural Science Foundation of China (Grant No. 31772723, No. 32002274, No. 32072872).

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Authors and Affiliations

  1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    Sun-Ting Ma, Yong-Shan Wang, Xiao-Li Wang, Xing-Xia Xia, Zhen-Wei Bi, Jing-Yu Wang, Yu-Mei Zhu, Wei Ouyang & Jing Qian

  2. Key Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture, Nanjing, 210014, China

    Sun-Ting Ma, Yong-Shan Wang, Xiao-Li Wang, Xing-Xia Xia, Zhen-Wei Bi, Jing-Yu Wang, Yu-Mei Zhu, Wei Ouyang & Jing Qian

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  1. Sun-Ting Ma
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  2. Yong-Shan Wang
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  8. Wei Ouyang
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Contributions

JQ, YSW, and WOY designed study; WOY, XLW, and XXX performed the experiments; ZWB and YMZ analyzed data; STM wrote the paper. All authors approved the version.

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Correspondence to Wei Ouyang or Jing Qian.

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Edited by Keizo Tomonaga.

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Ma, ST., Wang, YS., Wang, XL. et al. Mass spectrometry-based proteomic analysis of potential infectious bursal disease virus VP3-interacting proteins in chicken embryo fibroblasts cells. Virus Genes 57, 194–204 (2021). https://doi.org/10.1007/s11262-021-01828-x

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  • DOI: https://doi.org/10.1007/s11262-021-01828-x

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