Abstract

This study aimed at obtaining an extract enriched in phenolic compounds from the aerial parts of black wattle waste (Acacia mearnsii De Wild), characterization of its composition, and investigation of its antioxidant potential and preclinical toxicological profile. First, a phenolic compound enriched extract (PCEE) was obtained by sequential fractionation. Thereafter, the main classes of secondary metabolites present in PCEE were identified and quantified. The antioxidant, cytotoxic, and cytogenotoxic activities were evaluated using in vitro assays. Toxicological effects on locomotor function were assessed using in vivo open field and plus maze models. Cinnamic acid and coumarin were isolated from the PCEE and identified. In addition, high contents of phenolic compounds and flavonoids, responsible for the promising antioxidant activity, were quantified. The extract should a dose-dependent in vitro cytotoxicity in HaCaT cells. In the cytogenotoxicity bioassay, no differences in the mitotic index and micronuclei frequency were observed between PCEE and negative control groups. Also, there were no significant effects of the extract on the motor function. Taken together, these results show that the aerial parts of A. mearnsii can be used for obtaining bioactive compounds with the potential for promoting human and animal health.

Graphic Abstract

中文翻译：

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从森林固体废物黑荆芥的空中部分分离出有价值的生物活性化合物

摘要

这项研究的目的是从黑荆豆废料的地上部分提取一种富含酚类化合物的提取物（Acacia mearnsiiDe Wild），对其成分进行表征，并研究其抗氧化潜力和临床前毒理学特征。首先，通过顺序分馏获得富含酚类化合物的提取物（PCEE）。此后，鉴定并量化了PCEE中存在的主要次要代谢物类别。使用体外测定法评估抗氧化剂，细胞毒性和细胞遗传毒性活性。使用体内野外和迷宫模型评估了对运动功能的毒理学影响。从PCEE中分离并鉴定了肉桂酸和香豆素。此外，量化了酚类化合物和类黄酮的高含量，这些酚类化合物和类黄酮具有良好的抗氧化活性。提取物在HaCaT细胞中应具有剂量依赖性的体外细胞毒性。在细胞遗传毒性生物测定中，在PCEE和阴性对照组之间，没有观察到有丝分裂指数和微核频率的差异。同样，提取物对运动功能没有明显影响。综合来看，这些结果表明mearnsii可以用于获得具有促进人类和动物健康潜力的生物活性化合物。

图形摘要