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Isolation of Valuable Bioactive Compounds from Aerial Parts of Black Wattle, a Forest Solid Waste

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Abstract

This study aimed at obtaining an extract enriched in phenolic compounds from the aerial parts of black wattle waste (Acacia mearnsii De Wild), characterization of its composition, and investigation of its antioxidant potential and preclinical toxicological profile. First, a phenolic compound enriched extract (PCEE) was obtained by sequential fractionation. Thereafter, the main classes of secondary metabolites present in PCEE were identified and quantified. The antioxidant, cytotoxic, and cytogenotoxic activities were evaluated using in vitro assays. Toxicological effects on locomotor function were assessed using in vivo open field and plus maze models. Cinnamic acid and coumarin were isolated from the PCEE and identified. In addition, high contents of phenolic compounds and flavonoids, responsible for the promising antioxidant activity, were quantified. The extract should a dose-dependent in vitro cytotoxicity in HaCaT cells. In the cytogenotoxicity bioassay, no differences in the mitotic index and micronuclei frequency were observed between PCEE and negative control groups. Also, there were no significant effects of the extract on the motor function. Taken together, these results show that the aerial parts of A. mearnsii can be used for obtaining bioactive compounds with the potential for promoting human and animal health.

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Acknowledgements

The authors are thankful to Universidade Feevale for the infrastructure and financial support.

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Authors and Affiliations

  1. Graduate Studies Program in Toxicology and Toxicological Analysis, Universidade Feevale, Novo Hamburgo, RS, Brazil

    Ana Valéria de Gonçalves Prietsch, Lenise Maria Spies, Ronete Haubert, Allan José da Silva Nunes, Olyr Celestino Kreutz, Fernando Dal Pont Morisso, Rage W. Maluf, Angela Beatrice Dewes Moura, Patricia Ardenghi & Edna Sayuri Suyenaga

  2. Institute of Health Sciences, Universidade do Vale do Rio dos Sinos—UNISINOS, São Leopoldo, RS, Brazil

    Patrícia Weimer & Rochele Cassanta Rossi

  3. Graduate Program in Pharmaceutical Sciences, Universidade Federal do Rio Grande do Sul—UFRGS, Avenida Ipiranga, n. 2752, Porto Alegre, RS, 90610-000, Brazil

    Patrícia Weimer

  4. Cytotoxicity Laboratory, Institute of Health Sciences, Universidade Feevale, Novo Hamburgo, RS, Brazil

    Ana Luiza Ziulkoski

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  1. Ana Valéria de Gonçalves Prietsch
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  2. Patrícia Weimer
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Contributions

AVGP: Conceptualization, Methodology, Investigation, Software, Data curation, and Writing—original draft. PW: Formal analysis, Methodology, Software, Investigation, and Writing—original draft. LMS: Formal analysis, Methodology, and Investigation. RH: Formal analysis, Methodology, Visualization, and Investigation. AJSN, OCK, FDM, RWM, ABDM, ALZ, and PA: Investigation and Data curation. RCR: Methodology, Supervision, and Writing—review & editing. ESS: Conceptualization, Methodology, Investigation, Supervision, and Writing—review & editing.

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Correspondence to Patrícia Weimer.

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The authors declare that there are no conflicts of interest.

Ethical Approval

In vivo experimental procedures complied with all regulations for animal experimentation in Guide for the Care and Use of Laboratory Animals (National Research Council, 2011) and were previously approved by the Ethics Committee for Animal Research of Feevale University (Novo Hamburgo, RS, Brazil) under protocol number: 01.14.037, date of approval 01.07.2015.

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de Gonçalves Prietsch, A.V., Weimer, P., Spies, L.M. et al. Isolation of Valuable Bioactive Compounds from Aerial Parts of Black Wattle, a Forest Solid Waste. Waste Biomass Valor 12, 4953–4964 (2021). https://doi.org/10.1007/s12649-021-01355-8

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