Background and aims

Organic matter addition is an important management practice for maintaining soil fertility and the sustainability of artificial ecosystems. However, the effects of different types of organic matter on the microbial community in the perennial plant rhizosphere are not well understood.

Methods

A pot experiment was conducted to study how soil type and organic inputs influence bacterial community composition in the Populus rhizosphere. Plants were grown in the common forest soil (COM) and the poplar forest soil (POP), and three types of organic matter were applied. The rhizosphere soils were sampled after four months growth and rhizobacterial community was analyzed by 16S rRNA high-throughput sequencing.

Results

Organic inputs increased the alpha diversity in COM but not in POP. Both the soil type and organic inputs significantly influenced community structures. The different types of organic matter jointly enriched Ramlibacter, Sphingomonas and Adhaeribacter OTUs in the Populus rhizosphere. Amendment with organic matter improved network complexity and stability but had little effect on the species composition of the rhizosphere network at the phylum level.

Conclusions

Increased network stability was found in the Populus rhizosphere with organic amendments. These results highlight that organic matter input increased the diversity and network interactions in the Populus rhizosphere, indicated promoting species coexistence caused by resource availability improvement.

中文翻译：

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不同土壤类型输入的三种有机物下杨树根际不同的细菌群落组成

背景和目标

添加有机物是维持土壤肥力和人工生态系统可持续性的重要管理实践。然而，对多年生植物根际中不同类型有机物对微生物群落的影响尚不十分了解。

方法

进行了盆栽试验，以研究土壤类型和有机输入如何影响胡杨根际的细菌群落组成。在普通森林土壤（COM）和杨树森林土壤（POP）中种植了植物，并施用了三种类型的有机物。生长四个月后对根际土壤进行采样，并通过16S rRNA高通量测序对根际细菌群落进行分析。

结果

有机输入增加了COM中的alpha多样性，但没有增加POP中的alpha多样性。土壤类型和有机投入都显着影响了群落结构。杨树根际中不同类型的有机物共同富集红杆菌，鞘氨醇单胞菌和Adhaeribacter OTU 。对有机物的修正改善了网络的复杂性和稳定性，但对系统级根际网络的物种组成影响不大。

结论

在有机修饰下，在杨树根际发现网络稳定性增加。这些结果表明，有机物输入增加了杨树根际的多样性和网络相互作用，表明促进了资源可利用性的改善导致物种共存。