Ionic liquids (ILs) have been gaining widespread attention due to their plethora of applications. In particular, increasing studies are being carried out to enhance the biological applications of ILs. In this work, a newly synthesized peptide amphiphile comprised of tert-butyl (6-amino-1-((6-aminohexyl) amino)-1-oxohexan-2-yl) carbamate (TAOC) was conjugated with the peptide segment derived from laminin, YIGSR, and self-assembled to form nanofibers. The formed nanofibers were then blended with two separate ionic liquids, betainium bis(trifluoromethylsulfonyl)imide [Hbet][NTf₂] and 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide [C₄mim][NTf₂] to form nanohybrids. To the nanohybrids, collagen (Type IV) was incorporated to further enhance biocompatibility. Our results indicated that the imidazolium-based nanohybrids formed globular assemblies and displayed higher thermal stability and mechanical strength compared to [Hbet][NTf₂]-based nanocomposites. The binding interactions with the ionic liquids were probed by FTIR spectroscopy, DSC, TGA as well as predictive COSMO-RS studies, which indicated the key role of hydrogen bonding and hydrophobic interactions. Cell studies with neural cortical cells revealed that in both cases, the nanohybrids reduced cytotoxicity compared to the neat ionic liquids. Furthermore, axonal growths were observed. Such ionic liquid infused peptide nanohybrids, particularly the imidazolium-based nanohybrid gels may have potential for biological applications.

离子液体（IL）由于其用途广泛而受到广泛关注。特别地，正在进行越来越多的研究以增强IL的生物学应用。在这项工作中，由氨基甲酸叔丁基（6-氨基-1-（（（6-氨基己基）氨基）-1-氧己基-2-基）氨基甲酸酯（TAOC）组成的新合成的肽两亲物与层粘连蛋白，YIGSR，并自组装形成纳米纤维。然后将形成的纳米纤维与两种单独的离子液体，双（三氟甲基磺酰基）酰亚胺铍[Hbet] [NTf ₂ ]和1-丁基-3-甲基咪唑双（三氟甲基磺酰基）酰亚胺[C ₄ mim] [NTf ₂]形成纳米杂化物。对于纳米杂化物，掺入了胶原蛋白（IV型）以进一步增强生物相容性。我们的结果表明，与[Hbet] [NTf ₂ ]相比，基于咪唑鎓的纳米杂化物形成球状组件并显示出更高的热稳定性和机械强度。基于]的纳米复合材料。通过FTIR光谱，DSC，TGA以及预测性COSMO-RS研究探索了与离子液体的结合相互作用，这表明了氢键和疏水相互作用的关键作用。用神经皮质细胞进行的细胞研究表明，在两种情况下，与纯离子液体相比，纳米混杂物均降低了细胞毒性。此外，观察到轴突生长。此类离子液体注入的肽纳米杂化物，尤其是基于咪唑鎓的纳米杂化物凝胶可能具有生物学应用的潜力。