Abstract
Ionic liquids (ILs) have been gaining widespread attention due to their plethora of applications. In particular, increasing studies are being carried out to enhance the biological applications of ILs. In this work, a newly synthesized peptide amphiphile comprised of tert-butyl (6-amino-1-((6-aminohexyl) amino)-1-oxohexan-2-yl) carbamate (TAOC) was conjugated with the peptide segment derived from laminin, YIGSR, and self-assembled to form nanofibers. The formed nanofibers were then blended with two separate ionic liquids, betainium bis(trifluoromethylsulfonyl)imide [Hbet][NTf2] and 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide [C4mim][NTf2] to form nanohybrids. To the nanohybrids, collagen (Type IV) was incorporated to further enhance biocompatibility. Our results indicated that the imidazolium-based nanohybrids formed globular assemblies and displayed higher thermal stability and mechanical strength compared to [Hbet][NTf2]-based nanocomposites. The binding interactions with the ionic liquids were probed by FTIR spectroscopy, DSC, TGA as well as predictive COSMO-RS studies, which indicated the key role of hydrogen bonding and hydrophobic interactions. Cell studies with neural cortical cells revealed that in both cases, the nanohybrids reduced cytotoxicity compared to the neat ionic liquids. Furthermore, axonal growths were observed. Such ionic liquid infused peptide nanohybrids, particularly the imidazolium-based nanohybrid gels may have potential for biological applications.
Highlights
-
A new peptide amphiphile (PA) comprised of Boc-Lys conjugated with hexamethylene diamine and YIGSR was synthesized.
-
Role of the cationic component of [C4mim][NTf2] and [Hbet][NTf2] on binding interactions with the PA was explored.
-
The IL-Pas were integrated with collagen to enhance biocompatibility
-
The nanocomposite gels enhanced cell viability compared to neat ILs.
-
[C4mim][NTf2]-TAOC-YIGSR-collagen nanogels displayed higher viscosity and enhanced cell viability.
Similar content being viewed by others
References
Marrucho IM, Branco LC, Rebelo LP (2014) Ionic liquids in pharmaceutical applications. Annu Rev Chem Biomol Eng 5:527–546
Noritomi H, Minamisawa K, Kamiya R, Kato S (2011) Thermal stability of proteins in the presence of aprotic ionic liquids. J Biomed Sci Eng 4:94–99
Jovanovic-Santa S, Kojic V, Atlagic K, Tot A, Vranes M, Gadzuric S, Karaman M (2020) Anticancer and antimicrobial properties of imidazolium based ionic liquids with salicylate anion. J Serb Chem Soc 85:291–303
Ferraz R, Branco LC, Prudêncio C, Noronha JP, Petrovski Z (2011) Ionic liquids as active pharmaceutical ingredients. ChemMedChem 6:975–985
Sivapragasam M, Moniruzzaman M, Goto M (2016) Recent advances in exploiting ionic liquids for biomolecules: solubility, stability and applications. Biotechnol J 11:1000–1013
Hayakawa EH, Mochizuki E, Tsuda T, Akiyoshi K, Matsuoka H, Susumu K (2013) The effect of hydrophilic ionic liquids 1-ethyl-3-methylimidazolium lactate and choline lactate on lipid vesicle fusion. PloS ONE 8:e85467
Swatloski RP, Holbrey JD, Memon SB, Caldwell GA, Caldwell KA, Rogers RD (2004) Using caenorhabditis elegans to probe toxicity of 1-alkyl-3-methylimidazolium chloride based ionic liquids. Chem Commun 668–669
Bhosale VK, Kulkarni PS (2017) Ultrafast igniting, imidazolium based hypergolic ionic liquids with enhanced hydrophobicity. New J Chem 41:1250–1258
Zhao G, He F, Wu C, Li P, Li N, Deng J, Zhu G, Ren W, Peng Y (2018) Betaine in inflammation: mechanistic aspects and applications. Front Immunol 9:1070
Cho C-W, Pham TPT, Jeon Y-C, Yun YS (2008) Influence of anions on the toxic effects of ionic liquids to a phytoplankton Selenastrum capriconutum. Green Chem 10:67–72
Backer T, Mudring A-V (2012) Betaine chloride-betaine tetrachloidoferrate (III)—an ionic liquid related crystal structure governed by the pearson concept. Crystals 2:110–117
Deferm C, Luten J, Oosterhof H, Fransaer J, Binnemans K (2017) Purification of crude In (OH)3 using the functionalized ionic liquid betainium bis (trifluoromethylsulfonyl) imide. Green Chem 20:412–424
Freire MG, Neves CM, Marrucho IM, Coutinho JAP, Fernandes AM (2010) Hydrolysis of tetrafluoroborate and hexafluorophosphate counter ions in imidazolium-based ionic liquids. J Phys Chem A 114:3744–3749
Cooper EI, O’ Sullivan EJM (1992) New, stable, ambient molten salts. Proc Electrochem Soc 16:386–396
Frade RFM, Afonso C (2010) Impacts of ionic liquids in environment: an overview. Hum Expt Toxicol 29:1038–1054
Ruokonen S-K, Sanwald C, Sundvik M, Polnick S, Vyavharkar K, Dusa F, Holding AJ, King AW, Kilpelainen I, Lammerhofer M, Panula P, Wiedmer SK (2016) Effect of ionic liquids of zebrafish (Dan rerio) viability, behavior and histology. correlation between toxicity and ionic liquid aggregation. Environ Sci Technol 50:7116–7125
Frade RFM, Rosatella AA, Marques CS, Branco LC, Kulkarni PS, Mateus NMM, Afonso CAM, Duarte CMM (2009) Toxicological evaluation on human colon carcinoma cell line (Caco-2) of ionic liquids based on imidazolium, guanidium, ammonium, phosphonium, pyridinium and pyrrolidinium cations. Green Chem 11:1660–1665
Gupta MK, Khokar SM, Phillips DM, Sowards LA, Drummy LF, Kadakia MP, Naik RR (2007) Patterned silk films cast from ionic liquid solubilized fibroin as scaffolds for cell growth. Langmuir 23:1315–1319
Soni SK, Selvakannan PR, Bhargava SK, Bansal V (2012) Self-assembled histidine acid phosphatase nanocapsules in ionic liquid [BMIM][BF4] as functional templates for hollow metal nanparticles. Langmuir 28:10389–10397
Zhang Y, Chen X, Lan J, You J, Chen L (2009) Synthesis and biological applications of imidazolium-based polymerized ionic liquid as a gene delivery vector. Chem Biol Drug Des 74:282–288
Srivastava CM, Purwar R (2016) Fabrication of robust Antheraea assama fibroin nanofibrous mat using ionic liquid for skin tissue engineering. Mater Sci Eng C 68:276–290
Viau L, Tourné-Péteilh C, Devoisselle J, Viouxa A (2010) Ionogels as drug delivery system: one-step sol–gel synthesis using imidazolium ibuprofenate ionic liquid. Chem Commun 46:228–230
Kar T, Mandal SK, Das PK (2011) Organogel-hydrogel transformation by simple removal or inclusion of N-Boc protection. Chem Eur J 17:14952–14961
Saleh NT, Sohi AN, Esmaeili E, Karami S, Soleimanifar F, Nasoohi N (2019) Immobilized laminin-derived peptide can enhance expression of stemness markers in mesenchymal stem cells. Biotechnol Bioprocess Eng 24:876–884
Rao CJ, Raghavendran VK, Venkatesan KA, Nagarajan K, Srinivasan TG (2009) Thermochemical properties of some bis(fluoromethylsulfonyl)imide based room temperature ionic liquids. J Therm Anal Calorimet 97:937–943
Burrell AK, Del Sesto RE, Baker SN, McCleskey TM, Baker GA (2007) The large scale synthesis of pure imidazolium and pyrrolidinium ionic liquids. Green Chem 9:449–454
Klamt A (1995) Conductor-like screening model for real solvents: a new approach to the quantitative calculation of solvation phenomena. J Phys Chem 99:2224–2235
Klamt A, Jonas V, Bürger T, Lohrenz JC (1998) Refinement and parametrization of COSMO-RS. J Phys Chem A 102:5074–5085
Klamt A, Eckert F (2011) TmoleX3.1, COSMOlogic GmbH & Co. KG: Leverkusen, Germany
COSMOtherm, C3.0, release 1601, COSMOlogic GmbH & Co KG, http://www.cosmologic.de
Eckert F, Klamt A (2002) Fast solvent screening via quantum chemistry: COSMO-RS approach. AIChE J 48:369–385
Kurnia KA, Coutinho JAP (2013) Overview of the excess enthalpies of the binary mixtures composed of molecular solvents and ionic liquids and their modeling using COSMO-RS. Ind Eng Chem Res 52:13862–13874
Lee S, Trinh THT, Yoo M, Shin J, Lee H, Kim J, Hwang E, Lim Y-B (2019) Self-assembling peptides and their application in the treatment of diseases. Int J Mol Sci 20:5850
Subbalakshmi C, Manorama SV, Nagaraj R (2012) Self-assembly of short peptides composed of only aliphatic amino acids and a combination of aromatic and aliphatic amino acids. J Peptide Sci 18:283–292
Andukuri A, Minor WP, Kushwaha M, Anderson JM, Jun H-W (2010) Effects of endothelium mimicking self-assembled nanomatrices on cell adhesion and spreading of human endothelial cells and smooth muscle cells. Nanomedicine 6:289–297
Kushwaha M, Anderson JM, Bosworth CA, Andukur A, Minor WP, Lancaster JR, Anderson PG, Brott BC, Jun H-W (2010) A nitric oxide releasing, self-assembled peptide amphiphile matrix that mimics native endothelium for coating implantable cardiovascular devices. Biomaterials 31:1502–1508
Bowers J, Butts CP, Martin PJ, Vergara-Gutierrez MC (2004) Aggregation behavior of aqueous solutions of ionic liquids. Langmuir 20:2191–2198
Smirnova NA, Vanin AA, Safonova EA, Pukinsky IB, Anufrikov YA, Makarov AL (2009) Self-assembly in aqueous solutions of immidazolium ionic liquids and their mixtures with an anionic surfactant. J Coll Interface Sci 336:793–802
Singh G, Kang TS (2015) Ionic liquid surfactant mediated structural transitions and self-assembly of bovine serum albumin in aqueous media: effect of functionalization of ionic liquid surfactants. J Phys Chem B 119:10573–10585
Takekiyo T, Yamazaki K, Yamaguchi E, Abe H, Yoshimura Y(2012) High ionic liquid concentration-induced structural change of protein in aqueous solution: a case study of lysozyme. J Phys Chem B 116:11092–11097
Rawat K, Bohidar H (2012) Fluorescence and CD spectroscopic analysis of the α‐chymotrypsin stabilization by the ionic liquid, 1‐ethyl‐3‐methylimidazolium bis[(trifluoromethyl)sulfonyl amide. J Phys Chem B 116:11065–11074
Hwang J, San BH, Turner NJ, White LJ, Faulk DM, Badylak SF, Li Y, Yu SM (2017) Molecular assessment of collagen denaturation in decellularized tissues using a collagen hybridizing peptide. Acta Biomater 53:268–278
Liu S, Li Q, Li G (2019) Investigation of the solubility and dispersion degree of calf skin collagen in ionic liquids. J Leather Sci Eng 1: Article number: 11
Gupta H, Malik S, Sharma VK (2019) Excess molar enthalpies for [Bmmim][BF4] + [Bmim][BF4] or [Emim][BF4] + cyclopentanone or cyclohexanone mixtures. J Therm Anal Calorim 136:1383–1394
Adochitei A, Drochioiu G (2011) Rapid characterization of peptide secondary structure by FT-IR spectroscopy. Rev Roum Chim 56:783–791
Abood NA, Al-Askari M, Saeed BA (2012) Structures and vibrational frequencies of imidazole, benzimdazole and its 2-alkyl derivatives determined by DFT calculations. Basrah J Sci 30:119–131
Yamada T, Mizuno M (2018) Characteristic spectroscopic features because of cation−anion interactions observed in the 700−950 cm−1 range of infrared spectroscopy for various imidazolium-based ionic liquids. ACS Omega 3:8027–8035
Yamada T, Tominari Y, Tanaka S, Mizuno M (2017) Infrared spectroscopy of ionic liquids consisting of imidazolium cations with different alkyl chain lengths and various halogen or molecular anions with and without a small amount of water. J Phys Chem B 121:3121–3129
Khoshnoodi J, Pedchenko V, Hudson B (2008) Mammalian collagen IV. Microsc Res Technol 71:357–370
Belbachir K, Noreen R, Gouspillou G, Petibois C (2009) Collagen types analysis and differentiation by FTIR spectroscopy. Anal Bioanal Chem 395:829–837
Kuptsov AK, Zhizhin GN (1998) Handbook of Fourier transform raman and infrared spectra of polymers. Elsevier Science, Amsterdam, Netherlands. 45:1–581
Farlay D, Ducolos M-E, Gineyts E, Bertholon C, Viguet-Carrin S, Nallala J, Sockalingum GD, Bertrand D, Roger T, Hartmann DJ, Chapurlat R, Boivin G (2011) The ratio of 1660/1690 cm−1 measured by infrared microspectroscopy is not specific of enzymatic collagen cross-links in bone tissue. Plos One 6:328736
Ramasamy S, Ramadhar K (2015) A novel enzymatic method for preparation and characterization of collagen film from swim bladder of fish Rohu (Labeo rohita). Food Nutr Sci 6:1468–1478
Petibois C, Gouspillou G, Wehbe K, Delage J-P, Deleris G (2006) Analysis of type I and IV collagens by FTIR spectroscopy and imaging for a molecular investigation of skeletal muscle connective tissue. Anal Bioanal Chem 386:1961–1966
Fredlake CP, Crosthwaite JM, Hert DG, Aki SN, Brennecke JF (2004) Thermophysical properties of imidazolium-based ionic liquids. J Chem Eng Data 49:954–964
Badea E, Usacheva TR, Gatta GD (2015) The use of differential scanning calorimetry to characterize collagen deterioration in parchment. Rossijskikj Khimicheskij Z 59:28
Vander Hoogerstraete T, Onghena B, Binnemans K (2013) Homogeneous liquid–liquid extraction of rare earths with the betaine—betainium bis(trifluoromethylsulfonyl)imide ionic liquid system. Int J Mol Sci 14:21353–21377
Zychowicz et al. (2019) The collagen scaffold supports hiPSC-derived NSC growth and restricts hiPSC Front Biosci (Scholar edition) 11(1):105−121
Reddy RG, Zhang Z, Arenas MF, Blake DM (2003) Thermal stability and corrosivity evaluations of ionic liquids as thermal energy storage media. High Temp Mater Process 22:87–94
Dunn MS, Brophy TW (1932) Decomposition points of amino acids. J Biol Chem 99:221–229
Natarajan P, Sukthankar P, Changstrom J, Hollad CS, Barry S, Hunter WB, Sorensen CM, Tomich JM (2018) Synthesis and characterization of multifunctional branched amphiphilic peptide bilayer conjugated gold nanoparticles. ACS Omega 3:11071–11083
Avdibegovic D, Regadio M, Binnemans K (2017) Recovery of scandium (III) from diluted aqueous solutions by a supported ionic liquid phase (SLIP). RSC Adv 7:49664–49674
Wang B, Wang X, Lou W, Hao J (2010) Rheological and tribological properties of ionic liquid-based nanofluids containing functionalized multi-walled carbon nanotubes. J Phys Chem C 114:8749–8754
Dong K, Liu X, Dong H, Zhang X, Zhang S (2017) Multiscale studies on ionic liquids. Chem Rev 117:6636–6695
Mehta A, Rao JR, Fathima NN (2014) Effect of ionic liquids on the different hierarchical order of type I collagen. Colloids and Surfaces B: Biointerfaces 117:376–382
Tarannum A, Adams A, Blumich B, Fathima NN (2018) Impact of ionic liquids on the structure and dynamics of collagen. J Phys Chem B 122:1060−1065
Shkrob IA, Marin TW, Jensen MP (2014) Ionic liquid based separations of trivalent lanthanide and actinide ions. Ind Eng Chem Res 53:3641–3653
Tarannum A, Rao JR, Fathima NN (2018) Choline-based amino acid Ils-collagen interaction: enunciating its role in stabilization/destabilization phenomena. J Phys Chem B 122:1145–1151
Otto F, Gortz P, Fleischer W, Siebler M (2003) Cryopreserved rat cortical cells develop functional neuronal networks on microelectrode arrays. 128:173–181
Radošević K, Cvjetko M, Kopjar N, Novak R, Dumic J, Srcek VG (2013) In vitro cytotoxicity assessment of imidazolium ionic liquids: biological effects in fish channel catfish ovary (CCO) cell line. Ecotoxicol Environ Saf 92:112–118
Stepnowski P, Skladanowski AS, Ludwiczak A, Laczynska E (2004) Evaluating the cytotoxicity of ionic liquids using human cell line HeLa. Hum Exp Toxicol 23:513–517
Stolte S, Arning A, Bottin-Weber U, Muller A, Pitner WR, Welz-Biermann U, Jastroff B, Ranke J (2006) Anion effects on the cytotoxicity of ionic liquids. Green Chem 8:621–629
Waugh DT (2019) Fluoride exposure induces inibition of sodium and potassium-activated adenosine triphosphatase (Na+, K+- ATPase) enzyme activity: molecular mechanisms and implications for public health. Int J Environ Res Pub Health 16:1427
Moshikur RM, Chowdhury MR, Wakabayashi R, Tahara Y, Moniruzzaman M, Goto M (2018) Characterization and cytotoxicity evaluation of biocompatible amino acid esters used to convert salicylic acid into ionic liquids. Int J Pharm 546:31–38
Acknowledgements
MW and RD thank the Fordham University dean’s office for research grants for financial support of this work. IB thanks Dr. Karl Fath at the Queens College center of imaging cell and molecular biology for the use of the TEM. MT thanks the Office of Research and the Department of Natural Science for support.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
About this article
Cite this article
Whalen, M.S., Daso, R.E., Thomas, M.F. et al. Interactions of betainium and imidazolium-based ionic liquids with peptide amphiphiles and their implications in the formation of nanohybrid composite gels. J Sol-Gel Sci Technol 97, 488–504 (2021). https://doi.org/10.1007/s10971-020-05434-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10971-020-05434-5