Mining the gene of histidine content in barley grain helps with the breeding of functional barley varieties. The study constructed a recombinant inbred lines (RILs) containing 193 families derived from the cross between Ziguangmangluoerling (ZGMLEL) (♀) and Schooner No.3 (♂). The histidine (HIS) content in the grain of the mapping population and its parents were determined by an automatic amino acid analyzer. The HIS content of ZGMLEL was 0.53 mg/g. The grain HIS content of Schooner No. 3 was 0.21 mg/g, and the grain HIS content of population ranged from 0.23 to 0.54. Genetic linkage maps, including those of seven chromosomes of barley, were constructed by using 180 pairs of simple sequence repeat (SSR) markers, with a total genetic distance of 2671.03 cM and average marker spacing of 14.84 cM. Quantitative trait locus (QTL) IciMappingV3.3 was used to analyze QTL of HIS content in barley grains, and three QTLs were detected. Mapping results showed that the three loci were located on chromosomes 2H, 4H, and 7H, respectively. The major QTL with a contribution rate of 10.11% was located on barley chromosome 4H (HVBAMMGB84-BMAG0808). The additive effect is positive (0.025). Thus, it comes from the high-value parent ZGMLEL. Another major QTL with a contribution rate of 13.75% was located on barley chromosome 7H (GBM1303-GMS056). The minor QTL with a contribution rate of 6.01% was located on chromosome 2H (Scssr03381-Scssr07759). The additive effects of 4H and 7H QTLs were negative (− 0.02 and − 0.033). So, they came from the male parent Schooner. The results provided a reference for further fine mapping, cloning, and transformation of HIS genes in barley grains.

挖掘大麦籽粒中组氨酸含量的基因有助于功能性大麦品种的选育。该研究构建了一个重组自交系（RIL），该自交系包含193个光子，分别来自紫光曼格洛林（ZGMLEL）（3）和Schooner No. 3（♂）。用自动氨基酸分析仪测定作图群体及其亲本的籽粒中的组氨酸（HIS）含量。ZGMLEL的HIS含量为0.53mg / g。3号大篷车的谷物HIS含量为0.21 mg / g，种群的谷物HIS含量为0.23至0.54。遗传连锁图谱，包括大麦的七个染色体，通过使用180对简单序列重复（SSR）标记构建，总遗传距离为2671.03 cM，平均标记间距为14.84 cM。数量性状基因座（QTL）IciMappingV3。3用于分析大麦籽粒HIS含量的QTL，检测到3个QTL。映射结果显示这三个基因座分别位于2H，4H和7H染色体上。贡献率为10.11％的主要QTL位于大麦染色体4H（HVBAMMGB84-BMAG0808）上。加性效应为正（0.025）。因此，它来自高价值的父级ZGMLEL。大麦第7H染色体（GBM1303-GMS056）上有一个贡献率为13.75％的主要QTL。贡献率为6.01％的次要QTL位于2H染色体上（Scssr03381-Scssr07759）。4H和7H QTL的累加效应为负（-0.02和-0.033）。因此，他们来自男性父母Schooner。结果为大麦籽粒中HIS基因的进一步精细定位，克隆和转化提供了参考。