Knowledge of the behavior of corn starch during frozen storage is necessary to understand more complex systems. In the present study, ice recrystallization in corn starch (0.3% and 3%, w/w)/sucrose (40%, w/w) solution was investigated at −10 °C based on the theory of Ostwald ripening. The addition of corn starch to the sucrose solution increased the ice recrystallization (IR) rate constant. To explore the mechanism causing higher IR rate constant, fluorescence microscopy was used to analyze the distribution of corn starch molecules. Fluorescence micrograph showed corn starch distributed homogenously in the freeze-concentrated phase. Ice crystal size distribution assessment showed that at the same average radius, the addition of corn starch increased the standard deviation of ice crystal size distribution. The findings revealed that the addition of corn starch widened the distribution of ice crystal size, which may be the mechanism causing higher IR rate constant. To inhibit the ice recrystallization process, antifreeze protein type III (AFP III) was added to sucrose solutions with and without corn starch. In the presence of corn starch, 0.01-mg/mL AFP III was enough to significantly reduce the IR rate. Conversely, the samples without corn starch did not show a significant reduction in IR rate constant at the same AFP III concentration. The outcomes revealed that corn starch enhanced the activity of AFP III. The results of this study showed that corn starch increased the IR rate constant, and AFP III supplemented with corn starch was synergistically more efficient in retarding IR rate constant.

要了解更复杂的系统，必须了解冷冻保存期间玉米淀粉的行为。在本研究中，基于奥斯特瓦尔德熟化理论，在-10°C下研究了玉米淀粉（0.3％和3％，w / w）/蔗糖（40％，w / w）溶液中的冰重结晶。将玉米淀粉添加到蔗糖溶液中可增加冰的重结晶（IR）速率常数。为了探索引起较高的IR速率常数的机理，使用荧光显微镜分析了玉米淀粉分子的分布。荧光显微照片显示玉米淀粉在冷冻浓缩相中均匀分布。冰晶尺寸分布评估表明，在相同的平均半径下，玉米淀粉的添加增加了冰晶尺寸分布的标准偏差。研究结果表明，玉米淀粉的添加扩大了冰晶尺寸的分布，这可能是导致较高IR速率常数的机制。为了抑制冰的重结晶过程，将III型抗冻蛋白（AFP III）添加到有或没有玉米淀粉的蔗糖溶液中。在玉米淀粉的存在下，0.01 mg / mL AFP III足以显着降低IR率。相反，在相同的AFP III浓度下，不含玉米淀粉的样品的IR速率常数没有明显降低。结果表明，玉米淀粉增强了AFP III的活性。这项研究的结果表明，玉米淀粉提高了IR速率常数，而添加AFP III的玉米淀粉在延缓IR速率常数方面协同增效。