Anaerobic digestion of lignocellulosic biomass faces changes such as biomass floating and effluent discharge. To overcome these challenges, a unique removable cartridge anaerobic digester was built and tested using corn stover as the feedstock. Three operating methods differing in the number of cartridges and days of rotation were tested. The first method used three cartridges, with each cartridge being rotated every 7 days. The second and third methods employed four cartridges, with cartridges being rotated every 7 and 9–10 days, respectively. The retention time for methods 1, 2, and 3 was 21, 28, and 38 days, respectively. After observation spanning 1 year, it was found that the cartridge digester was capable of generating a stable amount of biogas for energy without biomass floating or effluent discharging issues. The average daily methane yield from each method was 7.57, 7.11, and 6.82 L/day/kg-VS, and the cumulative methane yield was 158.95, 199.04, and 259.00 L/kg-VS, respectively. Ammonium nitrogen and pH values were in normal ranges throughout the experiment. This study provided new knowledge in operating and optimizing this cartridge digester, which may be broadly used for the anaerobic digestion of lignocellulosic biomass in the near future.

木质纤维素生物质的厌氧消化面临生物质漂浮和出水排放等变化。为了克服这些挑战，我们建造了一个独特的可拆卸筒式厌氧消化器，并使用玉米秸秆作为原料进行了测试。测试了三种不同的墨盒数量和旋转天数的操作方法。第一种方法使用三个墨盒，每个墨盒每 7 天轮换一次。第二种和第三种方法使用四个墨盒，墨盒分别每 7 天和 9-10 天旋转一次。方法 1、2 和 3 的保留时间分别为 21、28 和 38 天。经过 1 年的观察，发现筒式蒸煮器能够产生稳定量的沼气作为能源，而不会出现生物质漂浮或污水排放问题。每种方法的平均日甲烷产量分别为7.57、7.11和6.82 L/day/kg-VS，累积甲烷产量分别为158.95、199.04和259.00 L/kg-VS。在整个实验过程中，铵态氮和 pH 值都在正常范围内。该研究为该筒式消化器的操作和优化提供了新知识，可在不久的将来广泛用于木质纤维素生物质的厌氧消化。