Matrilineal genetic diversity and relationship were investigated among eight morphologically identified native Ethiopian horse populations using polymorphisms in 46 mtDNA D-loop sequences (454 base pairs). The horse populations identified were Abyssinian, Bale, Borana, Horro, Kafa, Kundido feral horses, Ogaden and Selale. Mitochondrial DNA D-loop sequences were characterized by 15 variable sites that defined five different haplotypes. All genetic diversity estimates, including Reynolds’ linearized genetic distance, genetic differentiation (F_ST) and nucleotide sequence divergence (D_A), revealed a low genetic differentiation in native Ethiopian horse populations. However, Kundido feral and Borana domestic horses were slightly diverged from the rest of the Ethiopian horse populations. We also tried to shed some light on the matrilineal genetic root of native Ethiopian horses from a network constructed by combining newly generated haplotypes and reference haplotypes deposited in the GenBank for Eurasian type Turkish Anatolian horses that were used as a genetic conduit between Eurasian and African horse populations. Ninety-two haplotypes were generated from the combined Ethio-Eurasian mtDNA D-loop sequences. A network reconstructed from the combined haplotypes using Median-Joining algorithm showed that haplotypes generated from native Ethiopian horses formed separate clusters. The present result encourages further investigation of the genetic origin of native African horses by retrieving additional mtDNA sequences deposited in the GenBank for African and Eurasian type horses.

中文翻译：

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从线粒体DNA多态性推断埃塞俄比亚本地马种群（马属马）的遗传多样性和历史动态。

利用46 mtDNA D-loop序列（454个碱基对）中的多态性，研究了八个形态学鉴定的天然埃塞俄比亚马种群之间的母系遗传多样性及其关系。确定的马种群为阿比西尼亚，巴尔，博拉纳，霍罗，卡法，昆迪多野马，奥加登和塞拉莱。线粒体DNA D环序列的特征是定义了五个不同单倍型的15个可变位点。所有遗传多样性的估计值，包括雷诺的线性化遗传距离，遗传分化（F _ST）和核苷酸序列差异（D _A），表明在当地埃塞俄比亚马群中遗传分化低。但是，昆迪多野马和博拉纳的家养马与其他埃塞俄比亚马种略有不同。我们还试图通过结合新生成的单倍型和参考单倍型而建立的网络，阐明埃塞俄比亚本地马的母系遗传根，该单倍型和参考单倍型被用作欧亚型土耳其安那托利亚马的基因库，用作欧亚和非洲马之间的遗传通道。人口。从组合的Ethio-Eurasian mtDNA D-loop序列产生了九十二个单倍型。使用Median-Joining算法从组合单倍型重建的网络表明，从天然埃塞俄比亚马匹生成的单倍型形成了单独的簇。