Lipopeptide biosurfactants (LPBs) display unique properties with widespread therapeutic applications. Recently, the wound healing activity of LPBs has received more attention. However, few investigations have focused on the healing mechanisms of these compounds, particularly those that are associated with the angiogenic pathways. Therefore, the current study aimed at investigating the effect of a natural LPB produced by Acinetobacter. junii B6 on the angiogenic potential of human umbilical vein endothelial cells (HUVECs). The proliferation, migration, and three-dimensional tube forming potential of HUVECs were examined after treatment with LPB. By using western blotting, the expression of angiogenic-related genes such as hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) were also measured. LPB demonstrated no significant proliferative or cytotoxic effect on HUVECs at the concentrations lower than 600 µg/mL. Moreover, LPB at a concentration of 300 µg/mL caused a significant increase in the rates of migration and tube formation of HUVECs. LPB also enhanced the protein expression levels of HIF-1α and VEGF in HUVECs in a dose-dependent manner. The present results suggest that LPB might be considered a potential wound healing agent by modifying some angiogenic factors.

脂肽生物表面活性剂（LPB）具有独特的特性，具有广泛的治疗应用。近来，LPB的伤口愈合活性已受到更多关注。但是，很少有研究集中在这些化合物的愈合机制上，尤其是那些与血管生成途径有关的机制。因此，当前的研究旨在调查不动杆菌产生的天然LPB的作用。朱尼B6对人脐静脉内皮细胞（HUVEC）的血管生成潜力。用LPB处理后，检查了HUVEC的增殖，迁移和三维管形成潜能。通过蛋白质印迹，还测量了血管生成相关基因如缺氧诱导因子-1α（HIF-1α）和血管内皮生长因子（VEGF）的表达。LPB浓度低于600 µg / mL时，对HUVEC没有明显的增殖或细胞毒性作用。此外，浓度为300 µg / mL的LPB导致HUVEC的迁移速率和管形成显着增加。LPB还以剂量依赖的方式增强HUVEC中HIF-1α和VEGF的蛋白表达水平。