Skip to main content

Advertisement

SpringerLink
Log in

Lipopeptide Biosurfactant from Acinetobacter junii B6: A Promising Natural Surfactant for Promoting Angiogenesis

  • Published:
International Journal of Peptide Research and Therapeutics Aims and scope Submit manuscript

Abstract

Lipopeptide biosurfactants (LPBs) display unique properties with widespread therapeutic applications. Recently, the wound healing activity of LPBs has received more attention. However, few investigations have focused on the healing mechanisms of these compounds, particularly those that are associated with the angiogenic pathways. Therefore, the current study aimed at investigating the effect of a natural LPB produced by Acinetobacter. junii B6 on the angiogenic potential of human umbilical vein endothelial cells (HUVECs). The proliferation, migration, and three-dimensional tube forming potential of HUVECs were examined after treatment with LPB. By using western blotting, the expression of angiogenic-related genes such as hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) were also measured. LPB demonstrated no significant proliferative or cytotoxic effect on HUVECs at the concentrations lower than 600 µg/mL. Moreover, LPB at a concentration of 300 µg/mL caused a significant increase in the rates of migration and tube formation of HUVECs. LPB also enhanced the protein expression levels of HIF-1α and VEGF in HUVECs in a dose-dependent manner. The present results suggest that LPB might be considered a potential wound healing agent by modifying some angiogenic factors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Ayed HB, Bardaa S, Moalla D, Jridi M, Maalej H, Sahnoun Z, Rebai T, Jacques P, Nasri M, Hmidet NJPB (2015) Wound healing and in vitro antioxidant activities of lipopeptides mixture produced by Bacillus mojavensis A21. PROCESS BIOCHEM 50:1023–1030

    Article  Google Scholar 

  • Beck LS, Deguzman L, Lee WP, Xu Y, McFatridge LA, Amento EPJGF (1991) TGF-β1 accelerates wound healing: reversal of steroid-impaired healing in rats and rabbits. GROWTH FACTORS 5:295–304

    Article  CAS  Google Scholar 

  • Behnam B, Rezazadehkermani M, Ahmadzadeh S, Mokhtarzadeh A, Nematollahi-Mahani SN, Pardakhty A (2018) Microniosomes for concurrent doxorubicin and iron oxide nanoparticles loading; preparation, characterization and cytotoxicity studies. Artif Cells Nanomed Biotechnol 46:118–125

    Article  CAS  Google Scholar 

  • Bradford MMJAb (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Duarte C, Gudiña EJ, Lima CF, Rodrigues LR (2014) Effects of biosurfactants on the viability and proliferation of human breast cancer cells. AMB express 4:40

    Article  Google Scholar 

  • Fan L, Li J, Yu Z, Dang X, Wang K (2014) The hypoxia-inducible factor pathway, prolyl hydroxylase domain protein inhibitors, and their roles in bone repair and regeneration. BioMed research international 2014

  • Griffith CK, Miller C, Sainson RC, Calvert JW, Jeon NL, Hughes CC, George SC (2005) Diffusion limits of an in vitro thick prevascularized tissue. Tissue Eng 11:257–266

    Article  CAS  Google Scholar 

  • Gupta S, Raghuwanshi N, Varshney R, Banat IM, Srivastava AK, Pruthi PA, Pruthi VJB, Pharmacotherapy (2017) Accelerated in vivo wound healing evaluation of microbial glycolipid containing ointment as a transdermal substitute. BIOMED PHARMACOTHER 94:1186–1196

    Article  CAS  Google Scholar 

  • Hemlata B, Selvin J, Tukaram K (2015) Optimization of iron chelating biosurfactant production by Stenotrophomonas maltophilia NBS-11. Biocatal. Agric Biotechnol 4:135–143

    Article  Google Scholar 

  • Li J, Zhang YP, Kirsner RS (2003) Angiogenesis in wound repair: angiogenic growth factors and the extracellular matrix. MICROSC RES TECHNIQ 60:107–114

    Article  CAS  Google Scholar 

  • Lydon HL, Baccile N, Callaghan B, Marchant R, Mitchell CA, Banat, I.M.J.A.a., chemotherapy, (2017) Adjuvant antibiotic activity of acidic sophorolipids with potential for facilitating wound healing. Antimicrob. Agents Chemother 61:e02547-02516

    Article  CAS  Google Scholar 

  • McCOLL BK, Stacker SA, Achen MGJA (2004) Molecular regulation of the VEGF family–inducers of angiogenesis and lymphangiogenesis. APMIS 112:463–480

    Article  CAS  Google Scholar 

  • Mehrabani M, Najafi M, Kamarul T, Mansouri K, Iranpour M, Nematollahi M, Ghazi-Khansari M, Sharifi A (2015) Deferoxamine preconditioning to restore impaired HIF‐1α‐mediated angiogenic mechanisms in adipose‐derived stem cells from STZ‐induced type 1 diabetic rats. CELL PROLIFERAT 48:532–549

    Article  CAS  Google Scholar 

  • Miralles Socías A, Valdés E, Cristina S, Sastre-Coll A, Moranta Mesquida D, Asensio Landa AJ, Andrés G-SJ (2005) High affinity binding of β-carbolines to imidazoline I2B receptors and MAO-A in rat tissues: Norharman blocks the effect of morphine withdrawal on DOPA/noradrenaline synthesis in the brain. Eur J Pharmacol 518:234–242

    Article  Google Scholar 

  • Mirzamohammadi S, Mehrabani M, Tekiyehmaroof N, Sharifi A (2016a) Protective effect of 17β-estradiol on serum deprivation-induced apoptosis and oxidative stress in bone marrow-derived mesenchymal stem cells. Hum Exp Toxicol 35:312–322

    Article  CAS  Google Scholar 

  • Mirzamohammadi S, Nematollahi MH, Mehrbani M, Mehrabani M (2016b) Ferulic acid pretreatment could improve prognosis of autologous mesenchymal stromal cell transplantation for diabetic neuropathy. Cytotherapy 18:925–927

    Article  CAS  Google Scholar 

  • Naughton P, Marchant R, Naughton V, Banat, I.J.J.o.a.m., (2019) Microbial biosurfactants: current trends and applications in agricultural and biomedical industries. J. Appl. Microbiol. 127:12–28

    Article  CAS  Google Scholar 

  • Ohadi M, Dehghannoudeh G, Forootanfar H, Shakibaie M, Rajaee M (2018) Investigation of the structural, physicochemical properties, and aggregation behavior of lipopeptide biosurfactant produced by Acinetobacter junii B6. Int J Biol Macromol 112:712–719

    Article  CAS  Google Scholar 

  • Ohadi M, Dehghannoudeh G, Shakibaie M, Banat IM, Pournamdari M, Forootanfar H (2017a) Isolation, characterization, and optimization of biosurfactant production by an oil-degrading Acinetobacter junii B6 isolated from an Iranian oil excavation site. Biocatal Agric Biotechnol 12:1–9

    Article  Google Scholar 

  • Ohadi M, Forootanfar H, Dehghannoudeh G, Eslaminejad T, Ameri A, Shakibaie M, Adeli-Sardou M (2020) Antimicrobial, anti-biofilm, and anti-proliferative activities of lipopeptide biosurfactant produced by Acinetobacter junii B6. Microb Pathog 138:103806

    Article  CAS  Google Scholar 

  • Ohadi M, Forootanfar H, Rahimi HR, Jafari E, Shakibaie M, Eslaminejad T, Dehghannoudeh G (2017b) Antioxidant Potential and Wound Healing Activity of Biosurfactant Produced by Acinetobacter junii B6. Curr. Pharm. Biotechnol

  • Raeiszadeh M, Pardakhty A, Sharififar F, Farsinejad A, Mehrabani M, Hosseini-Nave H, Mehrabani M (2018) Development, physicochemical characterization, and antimicrobial evaluation of niosomal myrtle essential oil. Int J Pharm Sci 13:250

    Google Scholar 

  • Ruthenborg RJ, Ban J-J, Wazir A, Takeda N, Kim J-w (2014) Regulation of wound healing and fibrosis by hypoxia and hypoxia-inducible factor-1. Mol Cells 37:637

    Article  Google Scholar 

  • Semenza GL, Agani F, Booth G, Forsythe J, Iyer N, Jiang B-H, Leung S, Roe R, Wiener C, Yu A (1997) Structural and functional analysis of hypoxia-inducible factor 1. Kidney international 51:553–555

    Article  CAS  Google Scholar 

  • Stipcevic T, Piljac A, Piljac GJB (2006) Enhanced healing of full-thickness burn wounds using di-rhamnolipid. Burns 32:24–34

    Article  Google Scholar 

  • Stipcevic T, Piljac T, Isseroff RRJ.J.o.d.s., (2005) Di-rhamnolipid from Pseudomonas aeruginosa displays differential effects on human keratinocyte and fibroblast cultures. J. Dermatol. Sci 40:141–143

    Article  CAS  Google Scholar 

  • Tonnesen MG, Feng X, Clark RA (2000) Angiogenesis in wound healing, Journal of Investigative Dermatology Symposium Proceedings. Elsevier, pp. 40–46

  • Wright JA, Richards T, Srai SK (2014) The role of iron in the skin and cutaneous wound healing. Front Pharmacol 5:156

    Article  Google Scholar 

  • Yan L, Liu G, Zhao B, Pang B, Wu W, Ai C, Zhao X, Wang X, Jiang C, Shao D (2020) Novel Biomedical Functions of Surfactin A from Bacillus subtilis in Wound Healing Promotion and Scar Inhibition. J. Agric. Food Chem

  • Zhao R, Liang H, Clarke E, Jackson C, Xue MJIj.o.m.s., (2016) Inflammation in chronic wounds. Int. J. Mol. Sci. 17:2085

    Article  Google Scholar 

  • Zouari R, Moalla-Rekik D, Sahnoun Z, Rebai T, Ellouze-Chaabouni S, Ghribi-Aydi DJB, Pharmacotherapy (2016) Evaluation of dermal wound healing and in vitro antioxidant efficiency of Bacillus subtilis SPB1 biosurfactant. BIOMED PHARMACOTHER 84:878–891

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We are indebted to Kerman University of Medical Sciences (KMU) for contributing to the survey (97000960).

Author information

Authors and Affiliations

  1. Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

    Mehrnaz Mehrabani

  2. Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

    Mojdeh Esmaeili-Tarzi

  3. Pharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical Sciences, Kerman, Iran

    Hamid Forootanfar

  4. Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran

    Mohammad Hadi Nematollahi

  5. Department of Clinical Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

    Mohammad Hadi Nematollahi

  6. School of Biomedical Sciences, Faculty of Life & Health Sciences, University of Ulster, BT52 1SA, Coleraine, Northern Ireland, UK

    Ibrahim M. Banat

  7. Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

    Mandana Ohadi & Gholamreza Dehghannoudeh

Authors
  1. Mehrnaz Mehrabani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mojdeh Esmaeili-Tarzi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hamid Forootanfar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohammad Hadi Nematollahi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ibrahim M. Banat
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mandana Ohadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Gholamreza Dehghannoudeh
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Mehrnaz Mehrabani, Mandana Ohadi and Gholamreza Dehghannoudeh had significant involvement in the design, acquisition, analysis, and interpretation of the data. Hamid Forootanfar, Mohammad Hadi Nematollah, Mojdeh Esmaeili-Tarzi, Ibrahim M. Banat were significantly provided guidance in the overall design and delivery of the research. All authors were involved in revising the content, agree to take accountability for the integrity and accuracy of the work, and have read and approved the final manuscript.

Corresponding authors

Correspondence to Mandana Ohadi or Gholamreza Dehghannoudeh.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mehrabani, M., Esmaeili-Tarzi, M., Forootanfar, H. et al. Lipopeptide Biosurfactant from Acinetobacter junii B6: A Promising Natural Surfactant for Promoting Angiogenesis. Int J Pept Res Ther 27, 1197–1203 (2021). https://doi.org/10.1007/s10989-021-10160-9

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10989-021-10160-9

Keywords

Search

Navigation