Here, we are analyzing the comparative study of green-synthesized biogenic Caralluma tuberculata gold nanoparticles (Ca-AuNPs) and poly (ethylene glycol) methacrylate coated Ca-AuNPs nanocomposites (PEGMA-AuNPs), formed by hybridization of Ca-AuNPs with PEGMA. Both the virgin and polymer-capped materials (polymer hybrid) were physico-chemically characterized, using Fourier transform infrared spectroscopy (FTIR) that revealed physical interactions during coating without new peak, as PEGMA did not disturb the crystal structure of the AuNPs. However, a red shift of AuNPs from 450 to 520 nm was observed and the overall particle size of AuNPs was increased after PEGMA coating (50 to 100 nm). Furthermore, an efficient protocol of biomass enhancement of in vitro micropropagated Musa acuminate was achieved upon supplementation with Ca-AuNPs and PEGMA-Ca-AuNPs, in the Murashige and Skoog (MS) media in a dose-dependent manner. The maximum fresh, dry weight, and shoot length were achieved upon 80 ppm PEGMA-Ca-AuNPs supplementation. In the physiological analysis, higher chlorophyll content, abundant soluble sugar, and elevated moisture content also appeared upon 80 ppm PEGMA-AuNPs addition. Phytohormone analysis showed over-accumulation of IAA content, while on contrary, decreased MDA and H₂O₂ content, higher production of phenolics, flavonoids, phenylalanine ammonia lyase activity, and antioxidant activity was noticeable upon 80 ppm PEGMA-AuNPs supplementation. Moreover, enhanced antioxidant enzyme activities including CAT, SOD, POD, and APx were detected at 80 ppm PEGMA-Ca-AuNPs supplementation in MS media. It can be concluded that the PEGMA-AuNPs elicitation can be effectively used in plant tissue culture and agricultural industry, for sustainable promotion of biomass and secondary metabolic through modulating the physiological, biochemical, and bioactive antioxidants status and modifying cell wall properties.

在这里，我们正在分析通过Ca-AuNPs与PEGMA杂交形成的绿色合成的生物型结核菌金纳米颗粒（Ca-AuNPs）和聚（乙二醇）甲基丙烯酸酯包覆的Ca-AuNPs纳米复合材料（PEGMA-AuNPs）的比较研究。使用傅立叶变换红外光谱（FTIR）对原始材料和聚合物封端的材料（聚合物杂化物）进行了物理化学表征，揭示了涂层过程中的物理相互作用而没有新的峰，因为PEGMA不会干扰AuNPs的晶体结构。然而，观察到AuNP从450nm至520nm的红移，并且在PEGMA涂覆（50nm至100nm）之后AuNP的总粒径增加。此外，体外微繁提高生物量的有效协议在Murashige和Skoog（MS）培养基中以剂量依赖的方式补充Ca-AuNPs和PEGMA-Ca-AuNPs即可获得尖锐的穆萨舞。补充80 ppm PEGMA-Ca-AuNPs可获得最大的新鲜，干重和茎长。在生理分析中，添加80 ppm PEGMA-AuNPs时，也会出现较高的叶绿素含量，丰富的可溶性糖和较高的水分含量。植物激素分析显示IAA含量过多，相反，MDA和H ₂ O ₂降低当添加80 ppm PEGMA-AuNPs时，其含量，较高的酚类，类黄酮，苯丙氨酸氨裂合酶活性和抗氧化活性均显着。此外，在MS培养基中添加80 ppm PEGMA-Ca-AuNPs时，检测到增强的抗氧化酶活性，包括CAT，SOD，POD和APx。可以得出结论，PEGMA-AuNPs的诱导可有效地用于植物组织培养和农业，通过调节生理，生化和生物活性抗氧化剂的状态并改变细胞壁的性质来可持续地促进生物量和次生代谢。