The Homeodomain leucine zipper (HD-Zip) transcription factor family is one of the most important transcription factor families in the plant kingdom and it plays pivotal roles in plant development, growth as well as response to all kinds of stresses. However, the HD-Zip gene family has not been characterized in pineapple yet. Pineapple is one of the most economically important tropical fruit in the world. Here, we identified a total of 33 HD-Zip genes in pineapple by a genome-wide study. These 33 genes were divided into 4 subgroups and variably located on 14 chromosomes and one scaffold_1724. The gene structure and conserved motifs of these genes relatively share similar characteristics in a subfamily. We found at least two abiotic stress responding cis-elements in the promoter region of AcoHDzip genes, the light-responsive element is the most abundant cis-element that can be found in all the genes. Besides, transcriptome analysis showed the expression profile of 33 genes in different developmental stages. We identified the nine genes with higher expression in gynoecium and two genes with highly expressed in ovules and flowers respectively. Our studies will provide valuable information about the HD-Zip transcription factor family in pineapple, which would be useful for further functional studies in plant growth, plant development, and stress response.

同源域亮氨酸拉链（HD-Zip）转录因子家族是植物界最重要的转录因子家族之一，在植物发育，生长以及对各种胁迫的响应中起着关键作用。但是，HD-Zip基因家族尚未在菠萝中鉴定。菠萝是世界上最经济重要的热带水果之一。在这里，我们通过全基因组研究在菠萝中鉴定出总共33个HD-Zip基因。这33个基因被分为4个亚组，分别位于14条染色体和一个支架_1724上。这些基因的基因结构和保守基序在一个亚科中相对具有相似的特征。我们在AcoHDzip的启动子区域发现了至少两个非生物胁迫响应顺式元件基因中，光响应元件是所有基因中最多的顺式元素。此外，转录组分析显示了33个基因在不同发育阶段的表达谱。我们鉴定了在妇科中高表达的九个基因和在胚珠和花中高表达的两个基因。我们的研究将提供有关菠萝中HD-Zip转录因子家族的有价值的信息，这对于进一步进行植物生长，植物发育和胁迫响应的功能研究很有用。