In this study, sodium alginate film (Alg) was coated with electrospun collagen glue (Col) extracted from rabbit skin waste, loaded with different commercial antimicrobial agents (chitosan, AG425K and ZnONPs) and investigated in terms of morphological, structural and biological properties. The coated nanostructures were characterized using scanning electron microscopy coupled with the energy-dispersive X-ray (SEM/EDS), Attenuated Total Reflectance Fourier-Transform Infrared spectroscopy (ATR FT-IR), and Atomic Force Microscopy (AFM) tests. The cytotoxicity was investigated on murine L929 fibroblasts using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide salt (MTT) and lactate dehydrogenase (LDH) assays. Microbiological tests were performed against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 27853 standard strains. In vitro cell culture tests showed a good cytocompatibility of the coated nanostructured systems, except the sample loaded with ZnONPs, which exhibited a highly cytotoxic effect. Alg-Col-ZnONPs nanostructure inhibited the growth and multiplication of the Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 bacterial strains. The results of new coated nanostructures may be useful for the development of sustainable biomaterials in a circular economy, with bioactive properties for medical wound dressings.

中文翻译：

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基于藻酸盐和载有抗菌剂的电纺胶原蛋白的可持续涂层纳米结构

在这项研究中，海藻酸钠薄膜（Alg）涂有从兔皮废料中提取的静电纺丝胶原蛋白胶（Col），并负载了不同的商业抗菌剂（壳聚糖，AG425K和ZnONPs），并就形态，结构和生物学特性进行了研究。使用扫描电子显微镜，能量色散X射线（SEM / EDS），衰减全反射傅立叶变换红外光谱（ATR FT-IR）和原子力显微镜（AFM）测试，对涂覆的纳米结构进行了表征。使用3- [4,5-二甲基噻唑-2-基] -2,5-二苯基溴化四溴化铵盐（MTT）和乳酸脱氢酶（LDH）分析法研究了鼠L929成纤维细胞的细胞毒性。针对金黄色葡萄球菌ATCC 25923进行了微生物学测试，大肠杆菌ATCC 25922，铜绿假单胞菌ATCC 27853和白色念珠菌ATCC 27853标准菌株。体外细胞培养测试表明，经涂覆的纳米结构系统具有良好的细胞相容性，但载有ZnONP的样品除外，该样品表现出高度的细胞毒性作用。Alg-Col-ZnONPs纳米结构抑制了金黄色葡萄球菌ATCC 25923和大肠杆菌ATCC 25922细菌菌株的生长和繁殖。新的涂层纳米结构的结果可能对循环经济中具有生物活性的医用伤口敷料的可持续生物材料的开发有用。