Oxidative stress plays an important role in exacerbating neurodegenerative diseases, such as Alzheimer's disease, Parkinson’s disease, and Huntington’s disease. In a previous study, Lactobacillus plantarum 200655 was shown to possess probiotic and antioxidant potential. The current study aimed to evaluate the neuroprotective effects of heat-killed L. plantarum 200655. We incubated intestinal cells (HT-29) with heat-killed L. plantarum 200655 in a conditioned medium (CM) and found that the brain-derived neurotrophic factor (BDNF) mRNA level was elevated in the HT-29 cells and the CM contained high concentrations of BDNF. The CM protected neuroblastoma cells (SH-SY5Y) from hydrogen peroxide (H₂O₂)-induced toxicity. Moreover, the CM increased BDNF and tyrosine hydroxylase (TH) mRNA expression and significantly reduced the apoptosis-related Bax/Bcl-2 ratio in H₂O₂-treated SH-SY5Y cells. At the protein level, the CM resulted in downregulation of caspase-3. These results indicate that L. plantarum 200655 might be used as a prophylactic functional ingredient to prevent neurodegenerative disease.

氧化应激在加剧神经退行性疾病中起着重要作用，例如阿尔茨海默病、帕金森病和亨廷顿病。在之前的一项研究中，植物乳杆菌200655 被证明具有益生菌和抗氧化潜力。目前的研究旨在评估热灭活的植物乳杆菌200655的神经保护作用。我们将肠道细胞 (HT-29) 与热灭活的植物乳杆菌200655 在条件培养基 (CM) 中培养，发现脑源性神经营养HT-29 细胞中的因子 ( BDNF ) mRNA 水平升高，CM 含有高浓度的 BDNF。CM 保护神经母细胞瘤细胞 (SH-SY5Y) 免受过氧化氢 (H ₂ O ₂)-诱导的毒性。此外，CM 增加了 BDNF 和酪氨酸羟化酶 ( TH ) mRNA 的表达，并显着降低了H ₂ O ₂处理的 SH-SY5Y 细胞中与细胞凋亡相关的Bax / Bcl-2比率。在蛋白质水平上，CM 导致 caspase-3 下调。这些结果表明植物乳杆菌200655 可能用作预防神经退行性疾病的预防功能成分。