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Neuroprotective Effects of Heat-Killed Lactobacillus plantarum 200655 Isolated from Kimchi Against Oxidative Stress

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Abstract

Oxidative stress plays an important role in exacerbating neurodegenerative diseases, such as Alzheimer's disease, Parkinson’s disease, and Huntington’s disease. In a previous study, Lactobacillus plantarum 200655 was shown to possess probiotic and antioxidant potential. The current study aimed to evaluate the neuroprotective effects of heat-killed L. plantarum 200655. We incubated intestinal cells (HT-29) with heat-killed L. plantarum 200655 in a conditioned medium (CM) and found that the brain-derived neurotrophic factor (BDNF) mRNA level was elevated in the HT-29 cells and the CM contained high concentrations of BDNF. The CM protected neuroblastoma cells (SH-SY5Y) from hydrogen peroxide (H2O2)-induced toxicity. Moreover, the CM increased BDNF and tyrosine hydroxylase (TH) mRNA expression and significantly reduced the apoptosis-related Bax/Bcl-2 ratio in H2O2-treated SH-SY5Y cells. At the protein level, the CM resulted in downregulation of caspase-3. These results indicate that L. plantarum 200655 might be used as a prophylactic functional ingredient to prevent neurodegenerative disease.

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All data generated or analyzed during this study are included in this article.

Abbreviations

BDNF:

Brain-derived neurotrophic factor

Bcl-2:

B-cell lymphoma

Bax:

Bcl-2-associated X protein

TH:

Tyrosine hydroxylase

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Funding

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through the Innovational Food Technology Development Program (#119009-3), funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA).

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  1. Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029, Republic of Korea

    Min-Jeong Cheon, Na-Kyoung Lee & Hyun-Dong Paik

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Cheon, MJ., Lee, NK. & Paik, HD. Neuroprotective Effects of Heat-Killed Lactobacillus plantarum 200655 Isolated from Kimchi Against Oxidative Stress. Probiotics & Antimicro. Prot. 13, 788–795 (2021). https://doi.org/10.1007/s12602-020-09740-w

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