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Identification of QTLs associated with Sclerotinia blight resistance in peanut ( Arachis hypogaea L.)
Genetic Resources and Crop Evolution ( IF 1.6 ) Pub Date : 2020-09-15 , DOI: 10.1007/s10722-020-01012-4
Yuya Liang , John M. Cason , Michael R. Baring , Endang M. Septiningsih

Sclerotinia blight caused by Sclerotinia minor (Jagger) is a significant threat to peanut production; therefore varietal improvement toward this disease is needed. To date, there have been no reported quantitative trait locus (QTL) associated with Sclerotinia blight resistance in peanut. Hence, the objective of this study was to identify QTLs for Sclerotinia blight resistance. A total of 90 F2:6 recombinant inbred lines, derived from a released cultivar Tamrun OL07 and a breeding line Tx964117, were used as mapping population and field experiments were conducted in 2010, 2012 and 2018 at the Texas A&M AgriLife Research and Extension Center at Stephenville, Texas. A genetic map was developed using 1211 SNP markers based on double digest restriction-site associated DNA sequencing (ddRAD-Seq). In total, seven QTLs were identified, two QTLs from 2010 and five QTLs from 2018, with LOD score values of 3.2 to 7.2 and explaining 6.6–25.6% phenotypic variance. Among these QTLs, three were detected in common by WinQTLCart and R/qtl. Interestingly, one of the QTLs coincides with a previously reported peanut Leaf spot resistance QTL. The findings from this study not only provide insights into disease resistant QTLs in peanut but can also be used as potential targets for breeding programs to enhance Sclerotinia blight resistance through molecular breeding.



中文翻译:

花生中与核盘菌疫病抗性相关的QTLs的鉴定

小核盘菌(Jagger)引起的盘菌疫病是对花生生产的重大威胁。因此,需要对该病进行品种改良。迄今为止,尚无与花生核盘菌枯萎病抗性相关的定量性状位点(QTL)的报道。因此,本研究的目的是确定菌核疫病抗性的QTL。总共90 F 2:6重组自交系系来源于已发布的Tamrun OL07育种系和Tx964117育种系,用于作图种群,并于2010年,2012年和2018年在德克萨斯州斯蒂芬维尔的德克萨斯A&M农业生命研究和推广中心进行了田间试验。基于双酶切限制性位点相关的DNA测序(ddRAD-Seq),使用1211个SNP标记开发了遗传图谱。总共确定了7个QTL,2010年的2个QTL和2018年的5个QTL,LOD得分在3.2到7.2之间,表型差异为6.6-25.6%。在这些QTL中,WinQTLCart和R / qtl共同检测到三个。有趣的是,其中一个QTL与先前报道的花生叶斑病抗性QTL吻合。

更新日期:2020-09-15
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