Abstract
Sclerotinia blight caused by Sclerotinia minor (Jagger) is a significant threat to peanut production; therefore varietal improvement toward this disease is needed. To date, there have been no reported quantitative trait locus (QTL) associated with Sclerotinia blight resistance in peanut. Hence, the objective of this study was to identify QTLs for Sclerotinia blight resistance. A total of 90 F2:6 recombinant inbred lines, derived from a released cultivar Tamrun OL07 and a breeding line Tx964117, were used as mapping population and field experiments were conducted in 2010, 2012 and 2018 at the Texas A&M AgriLife Research and Extension Center at Stephenville, Texas. A genetic map was developed using 1211 SNP markers based on double digest restriction-site associated DNA sequencing (ddRAD-Seq). In total, seven QTLs were identified, two QTLs from 2010 and five QTLs from 2018, with LOD score values of 3.2 to 7.2 and explaining 6.6–25.6% phenotypic variance. Among these QTLs, three were detected in common by WinQTLCart and R/qtl. Interestingly, one of the QTLs coincides with a previously reported peanut Leaf spot resistance QTL. The findings from this study not only provide insights into disease resistant QTLs in peanut but can also be used as potential targets for breeding programs to enhance Sclerotinia blight resistance through molecular breeding.
Similar content being viewed by others
References
Baird NA et al (2008) Rapid SNP Discovery and Genetic Mapping Using Sequenced RAD Markers. PLoS ONE 3:e3376. https://doi.org/10.1371/journal.pone.0003376
Baring MR et al (2006) Registration of ‘Tamrun OL07’ Peanut. Crop Sci 46:2721–2722
Bennett RS, Chamberlin KD, Damicone JP (2018) Sclerotinia Blight Resistance in the US Peanut Mini-Core Collection. Crop Sci 58:1306–1317. https://doi.org/10.2135/cropsci2017.09.0591
Bernier J, Kumar A, Ramaiah V, Spaner D, Atlin G (2007) A Large-Effect QTL for Grain Yield under Reproductive-Stage Drought Stress in Upland Rice. Crop Sci 47:507–516. https://doi.org/10.2135/cropsci2006.07.0495
Bertioli DJ et al (2016) The genome sequences of Arachis duranensis and Arachis ipaensis, the diploid ancestors of cultivated peanut. Nat Genet 48:438–446. https://doi.org/10.1038/ng.3517
Broman KW, Wu H, Sen Ś, Churchill GA (2003) R/qtl: QTL mapping in experimental crosses. Bioinformatics 19:889–890
Buerstmayr H, Ban T, Anderson JA (2009) QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: a review. Plant Breed 128:1–26. https://doi.org/10.1111/j.1439-0523.2008.01550.x
Butzler TM, Bailey J, Beute Marvin K (1998) Integrated management of sclerotinia blight in peanut: utilizing canopy morphology, mechanical pruning, and fungicide timing. Plant Dis 82:1312–1318. https://doi.org/10.1094/PDIS.1998.82.12.1312
Chenault KD, Melouk HA, Payton ME (2005) Field Reaction to Sclerotinia Blight among Transgenic Peanut Lines Containing Antifungal Genes. Crop Sci 45(2):511–515
Chenault KD, Melouk HA, Payton ME (2006) Effect of Sclerotinia minor infection loci on peanut production parameters Peanut Sci 33:36–40
Chenault KD, Maas AL, Damicone JP, Payton ME, Melouk HA (2008) Discovery and characterization of a molecular marker for Sclerotinia minor (Jagger) resistance in peanut. Euphytica 166:357–365. https://doi.org/10.1007/s10681-008-9816-0
Chenault KD, Melouk HA, Payton ME (2009) Evaluation of the U.S. peanut mini core collection using a molecular marker for resistance to Sclerotinia minor Jagger. Euphytica 172:109–115. https://doi.org/10.1007/s10681-009-0065-7
Clevenger J et al (2017) Genome-wide SNP Genotyping Resolves Signatures of Selection and Tetrasomic Recombination in Peanut. Mol Plant 10:309–322. https://doi.org/10.1016/j.molp.2016.11.015
Clevenger J et al (2018) Mapping late leaf spot resistance in peanut (Arachis hypogaea) using QTL-seq reveals markers for marker-assisted selection front. Plant Sci 9:83. https://doi.org/10.3389/fpls.2018.00083
Corwin JA, Kliebenstein DJ (2017) Quantitative Resistance: more Than Just Perception of a Pathogen. Plant Cell 29:655–665. https://doi.org/10.1105/tpc.16.00915
Crutcher FK, Henry-Gregory MA, Wilkinson HH, Duke SE, Wheeler T, Kenerley CM (2018) Characterization of sclerotinia minor populations in Texas peanut fields. Plant Pathol. https://doi.org/10.1111/ppa.12792
Dixit S, Yadaw RB, Mishra KK, Kumar A (2017) Marker-assisted breeding to develop the drought-tolerant version of Sabitri, a popular variety from Nepal. Euphytica. https://doi.org/10.1007/s10681-017-1976-3
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull
Faske T, Emerson M, Hurd K (2014) First report of sclerotinia blight of peanut caused by sclerotinia minor in arkansas. Plant Dis 98:1013
Ferguson ME, Burow MD, Schulze SR, Bramel PJ, Paterson AH, Kresovich S, Mitchell S (2004) Microsatellite identification and characterization in peanut (A. hypogaea L.). Theor Appl Genet 108:1064–1070. https://doi.org/10.1007/s00122-003-1535-2
Glazebrook J (2005) Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu Rev Phytopathol 43:205–227
Goldman JJ, Smith OD, Simpson CE, Melouk HA (1995) Progress in breeding Sclerotinia blight-resistant runner-type peanut. Peanut Sci. 22(2):109–113
Gomez Selvaraj M, Narayana M, Schubert AM, Ayers JL, Baring MR, Burow MD (2009) Identification of QTLs for pod and kernel traits in cultivated peanut by bulked segregant analysis. Electron J Biotechn. https://doi.org/10.2225/vol12-issue2-fulltext-13
Grichar WJ, Woodward JE (2016) Fungicides and application timing for control of early leafspot, southern blight, and sclerotinia blight of peanut. Intern J Agron 2016:1–7. https://doi.org/10.1155/2016/1848723
Han S et al (2018) A SNP-based linkage map revealed QTLs for resistance to early and late leaf spot diseases in peanut (Arachis hypogaea L). Front Plant Sci. 9:1012. https://doi.org/10.3389/fpls.2018.01012
Henry A, Dixit S, Mandal NP, Anantha MS, Torres R, Kumar A (2014) Grain yield and physiological traits of rice lines with the drought yield QTL qDTY121 showed different responses to drought and soil characteristics in upland environments. Funct Plant Biol 41:1. https://doi.org/10.1071/fp13324
Hu J, Telenko DEP, Phipps PM, Grabau EA (2015) Comparative susceptibility of peanut genetically engineered for sclerotinia blight resistance to non-target peanut pathogens. Europ J of Plant Pathol 145:177–187. https://doi.org/10.1007/s10658-015-0831-4
Liang Y, Baring M, Wang S, Septiningsih EM (2017) Mapping QTLs for leafspot resistance in peanut using SNP-based next-generation sequencing markers. Plant Breed Biotech 5:115–122. https://doi.org/10.9787/PBB.2017.5.2.115
Liang Y, Baring MR, Septiningsih EM (2018) Mapping of quantitative trait loci for yield and grade related traits in peanut (Arachis hypogaea L.) using high-resolution SNP markers. Plant Breed Biotech 6:454–462. https://doi.org/10.9787/PBB.2018.6.4.454
Mallikarjuna N, Varshney RK (2014) Genetics, genomics and breeding of peanuts.Genetics, genomics and breeding of peanut: an introduction. CRC Press, Boca Raton
McCough SR, Doerge RW (1995) QTL mapping in rice. Trends Genet 11:482–487. https://doi.org/10.1016/S0168-9525(00)89157-X
Melzer MS, Smith EA, Boland GJ (1997) Index of plant hosts of Sclerotinia minor. Canadian J Plant Pathol 19:272–280. https://doi.org/10.1080/07060669709500523
Peterson BK, Weber JN, Kay EH, Fisher HS, Hoekstra HE (2012) Double Digest RADseq: an inexpensive method for de Novo SNP discovery and genotyping in model and non-model species. PLoS ONE 7:e37135. https://doi.org/10.1371/journal.pone.0037135
Phipps P (1995) An assessment of environmental conditions preceding outbreaks of Sclerotinia blight of peanut in Virginia. Peanut Sci 22:90–93
Porter DM, Melouk HA (1997) Compendium of peanut diseases, 2nd edn. American Phytopathological Society, St. Paul
Varshney RK et al (2009) The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L). Theor Appl Genet 118:1. https://doi.org/10.1007/s00122-008-0933-x
Wang S, Basten CJ, Zeng Z-B (2012) Windows QTL Cartographer 25. Department of Statistics, North Carolina State University, Raleigh
Wu Y, Bhat PR, Close TJ, Lonardi S (2008) Efficient and accurate construction of genetic linkage maps from the minimum spanning tree of a graph. PLoS Genet 4:e1000212. https://doi.org/10.1371/journal.pgen.1000212
Yol E, Upadhyaya HD, Uzun B (2014) Molecular diagnosis to identify new sources of resistance to sclerotinia blight in groundnut (Arachis hypogaea L). Euphytica 203:367–374. https://doi.org/10.1007/s10681-014-1282-2
Funding
This research was supported in part by a grant from Texas A&M AgriLife Research, the Texas Peanut Producers Board, the National Peanut Board, and the National Institute of Food and Agriculture, U. S. Department of Agriculture, Hatch Project 1009300.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare that they have no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Liang, Y., Cason, J.M., Baring, M.R. et al. Identification of QTLs associated with Sclerotinia blight resistance in peanut (Arachis hypogaea L.). Genet Resour Crop Evol 68, 629–637 (2021). https://doi.org/10.1007/s10722-020-01012-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10722-020-01012-4