The production of biofuels from lignocellulosic biomass includes a pretreatment step to alter the biomass structure and facilitate the enzymatic degradation of the polymers to obtain assimilable compounds. In this study, agave bagasse (AB) was used as a feedstock for obtaining methane, for which AB was pretreated with steam explosion and enzymatically hydrolyzed. The pretreatment conditions corresponded to severity factors (SFs) within a range from 1.65 to 2.89, while enzymatic hydrolysis was performed with enzyme loads of Cellic CTec2 within a range from 0.12 to 3.6 mg_protein g⁻¹_AB. The best global yields (including pretreatment and enzymatic hydrolysis) of total carbohydrates (TCs), glucose (GLU), xylose (XYL), and chemical oxygen demand (COD) were 0.7 g TC g⁻¹_AB, 0.12 g GLU g⁻¹_AB, 0.03 g XYL g⁻¹_AB, and 0.20 g O₂ g⁻¹_AB obtained using 2.4 mg_protein g⁻¹_AB of Cellic CTec2 with agave bagasse pretreated with an SF of 2.41. The contribution of pretreatment to the global TC yield ranged from 13 to 34% for the different systems evaluated. The biochemical potential of methane (BMP) of hydrolysates (pretreatment at SF 2.41 and 2.4 mg_protein g⁻¹_AB of Cellic CTec2) was 0.284 ± 0.02 in NL CH₄ g⁻¹ COD with a COD removal of 78.4 ± 1.3. This BMP value was 40% higher than the BMP obtained in the system without enzymatic hydrolysis, indicating the impact of this step on conversion to biomethane. The results at the BMP level indicated the potential of this residue for biofuel production.

由木质纤维素生物质生产生物燃料包括预处理步骤，以改变生物质结构并促进聚合物的酶促降解以获得可同化的化合物。在这项研究中，将龙舌兰蔗渣（AB）用作获得甲烷的原料，将其用蒸汽爆炸法预处理并进行酶水解。预处理条件对应的严重性因子（SFs）在1.65至2.89的范围内，而Cellic CTec2的酶负荷在0.12至3.6 mg_蛋白质g ^-1 _AB范围内进行酶促水解。总碳水化合物（TCs），葡萄糖（GLU），木糖（XYL）和化学需氧量（COD）的最佳全球产量（包括预处理和酶促水解）为0.7 g TC g^-1 _AB，0.12 g GLU g ^-1 _AB，0.03 g XYL g ^-1 _AB和0.20 g O ₂ g ^-1 _AB是使用2.4 mg_蛋白质g ^-1 _AB的Cellic CTec2和龙舌兰蔗渣获得的，SF预处理为2.41 。对于所评估的不同系统，预处理对全球TC产量的贡献范围为13％至34％。在NL CH ₄ g ^-1中，水解产物（SF 2.41和2.4 mg_蛋白g ^-1 _AB Cellic CTec2的预处理）的水解物甲烷（BMP）的生化潜力为0.284±0.02COD去除率为78.4±1.3。该BMP值比在没有酶水解的系统中获得的BMP高40％，表明该步骤对转化为生物甲烷的影响。BMP水平的结果表明该残留物具有生产生物燃料的潜力。