Summary Knowledge of the sperm–oocyte ratio in fish fertilization serves as the basis for studies on artificial reproduction and gamete manipulation. The aim of this study was to determine the minimum insemination dose for Brycon amazonicus oocyte fertilization. Female and male gametes were used and tested with the following doses of spermatozoa oocyte–1 ml–1: 10,000, 20,000, 40,000, 60,000 and 80,000 (in triplicate). Fertilization rates were calculated and estimated from the regression equation by applying the segmented regression model ‘Linear Response Plateau’ to determine the appropriate proportion of gametes. Based on the equation Ŷ = 14.3415 + 0.0007836X, the fertilization rate increased up to 63.34% as it reached a plateau with a proportion of 62,524 spermatozoa oocyte–1 ml–1, which is the minimum insemination dose recommended for artificial insemination of the species.

中文翻译：

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Brycon amazonicus (Teleostei: Characidae) 人工受精的授精剂量

总结 了解鱼类受精中的精卵比是人工繁殖和配子操作研究的基础。本研究的目的是确定最低授精剂量野马铃薯卵母细胞受精。使用雌性和雄性配子并用以下剂量的精子卵母细胞进行测试–1毫升–1：10,000、20,000、40,000、60,000 和 80,000（一式三份）。通过应用分段回归模型“线性响应高原”来确定配子的适当比例，从回归方程计算和估计受精率。根据方程 Ŷ = 14.3415 + 0.0007836X，受精率增加到 63.34%，因为它达到了 62,524 个精子卵母细胞的比例–1毫升–1，这是该物种人工授精推荐的最小授精剂量。