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Inseminating dose for the artificial fertilization of Brycon amazonicus (Teleostei: Characidae)

Published online by Cambridge University Press:  15 January 2021

Rosilane Gomes de Souza de Oliveira Open the ORCID record for Rosilane Gomes de Souza de Oliveira [Opens in a new window]  and
Marle Angélica Villacorta-Correa
Rosilane Gomes de Souza de Oliveira*
Affiliation:
Federal University of Amazonas (UFAM), Faculty of Agricultural Sciences, Department of Fisheries Sciences, Manaus, Amazonas, Brazil
Marle Angélica Villacorta-Correa
Affiliation:
Federal University of Amazonas (UFAM), Faculty of Agricultural Sciences, Department of Fisheries Sciences, Manaus, Amazonas, Brazil
*
Author for correspondence: Rosilane Gomes de Souza de Oliveira. Federal University of Amazonas (UFAM), Faculty of Agricultural Sciences, Department of Fisheries Sciences, Av. Gen. Rodrigo Octávio Jordão Ramos, 3000, CEP: 69077-000, Manaus, Amazonas, Brazil. Tel: +55 923305 1797. Fax: +55 923305 1797. E-mail: rosilanegso@gmail.com
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Summary

Knowledge of the sperm–oocyte ratio in fish fertilization serves as the basis for studies on artificial reproduction and gamete manipulation. The aim of this study was to determine the minimum insemination dose for Brycon amazonicus oocyte fertilization. Female and male gametes were used and tested with the following doses of spermatozoa oocyte–1 ml–1: 10,000, 20,000, 40,000, 60,000 and 80,000 (in triplicate). Fertilization rates were calculated and estimated from the regression equation by applying the segmented regression model ‘Linear Response Plateau’ to determine the appropriate proportion of gametes. Based on the equation Ŷ = 14.3415 + 0.0007836X, the fertilization rate increased up to 63.34% as it reached a plateau with a proportion of 62,524 spermatozoa oocyte–1 ml–1, which is the minimum insemination dose recommended for artificial insemination of the species.

Keywords

Artificial reproductionFertilization rateMatrinxãOocytesSpermatozoa
Type
Short Communication
Information
Zygote , Volume 29 , Issue 3 , June 2021 , pp. 252 - 255
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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