This study examined the microbicidal activity of ultraviolet (UV)-C_185–256-nm irradiance (robot 1) and ozone generated at UV-C_185-nm by low-pressure mercury vapor lamps (robot 2) adapted to mobile robotic devices for surface decontamination, which was achieved in less than 1 h. Depending on their wall structure and outer envelopes, many microorganisms display different levels of resistance to decontaminating agents. Thus, the need for novel disinfection approaches is further exacerbated by the increased prevalence of multidrug-resistant bacteria, as well as the potential of novel microorganisms, with the ability to cause disease outbreaks. To set up a rapid and effective approach for microorganisms propagation prevention, we focused on the effects of UV-C and ozone on a distinct microorganism survival ratio. A set of microorganisms, including Escherichia coli, Micrococcus luteus, Saccharomyces cerevisiae, Trichoderma harzianum, and Bacillus subtilis, were used to evaluate the disinfection power of UV-C and UV-C plus ozone generating robots. UV-C disinfection can be suited to ad hoc tasks, is easy to operate, requires low maintenance, does not have the need for the storage of dangerous chemicals, and does not produce by-products that may affect human health and the environment. The robotic cumulative irradiation technology developed (fluence accumulated values of 2.28 and 3.62 mJ cm⁻², for robot 1 and 2, respectively), together with the production of ozone (with a maximum peak of 0.43 ppm) capable of reaching UV-C shaded surfaces, and analyzed in the current study, despite being designed for the need to reduce the risk of epidemic outbreaks in real-life scenarios, represents a versatile tool that could be employed for air and surface disinfection within many circumstances that are faced daily.

中文翻译：

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UV-C和UV-C Plus臭氧产生机器人消毒潜能的微生物学评估

这项研究检查了紫外线（UV）-C _185-256-nm辐照度（机器人1）和在紫外线-C _185-nm处产生的臭氧的杀菌活性低压汞蒸气灯（机器人2）适用于表面污染的移动机器人设备，在不到1小时的时间内即可实现。根据它们的壁结构和外壳，许多微生物对去污剂表现出不同水平的抗性。因此，对多药耐药细菌的普及以及具有引起疾病暴发的能力的新型微生物的潜力进一步加剧了对新型消毒方法的需求。为了建立一种快速有效的预防微生物繁殖的方法，我们集中于UV-C和臭氧对不同微生物存活率的影响。一整套微生物，包括大肠杆菌，黄球菌，酿酒酵母，哈茨木霉和枯草芽孢杆菌用于评估UV-C和UV-C以及臭氧产生机器人的消毒能力。UV-C消毒可适合临时任务，易于操作，维护成本低，不需要存储危险化学品，并且不会产生可能影响人类健康和环境的副产品。开发了机器人累积辐照技术（注量累积值为2.28和3.62 mJ cm ^-2，分别用于机器人1和2），以及能够到达UV-C阴影表面的臭氧（最大峰值为0.43 ppm）的产生，并且尽管旨在减少臭氧排放，但仍在本研究中进行了分析。在现实生活中，流行病暴发的风险代表了一种多功能工具，可以在每天面对的许多情况下用于空气和表面消毒。