Isolation of novel actinobacteria from unexplored habitats as potential sources of novel drug leads has utmost importance. During the course of screening arid soil samples for novel actinobacteria, strain H3C3^T was isolated from Malatya, Turkey and its taxonomic position was revealed by a genome-based polyphasic approach. Pairwise sequence comparison of the 16S rRNA gene showed that the strain is closely related to Actinomadura fibrosa JCM 9371^T with sequence identity level of 99.0%. Comparative genome analyses based on digital DNA-DNA hybridization and average nucleotide identity indicated that strain H3C3^T represents a novel species within the genus Actinomadura. The strain has typical characteristics of the genus Actinomadura, i.e. meso-diaminopimelic acid as diagnostic amino acid; galactose, glucose, madurose and ribose as whole-cell sugars. Major menaquinones detected were MK-9(H₆), MK-9(H₈) and polar lipids were diphosphatidylglycerol, phosphatidylinositol, glycophospholipid and unknown phospholipid and lipids. Its genome size is approximately 10.2 Mb with G+C content of 71.6%. Further genomic analyses of strain H3C3^T indicated its high potential for novel biosynthetic gene clusters coding for various chemical structures. On the basis of phenotypic and phylogenetic analyses, strain H3C3^T represents a novel species of the genus Actinomadura, for which Actinomadura rubrisoli sp. nov. is proposed, and it holds high promise for novel biosynthetic metabolites of value to biopharmaceutical industry.

从未开发的栖息地中分离出新的放线菌作为潜在的新药线索来源至关重要。在筛选新型放线菌的干旱土壤样品的过程中，从土耳其马拉蒂亚分离出H3C3 ^T菌株，并通过基于基因组的多相方法揭示了其分类位置。16S rRNA基因的成对序列比较表明，该菌株与Actinomadura fibrosa JCM 9371 ^T亲缘关系密切，序列同一性水平为99.0%。基于数字 DNA-DNA 杂交和平均核苷酸同一性的比较基因组分析表明菌株 H3C3 ^T代表Actinomadura属内的一个新物种. 该菌株具有马杜拉放线菌属的典型特征，即内消旋二氨基庚二酸作为诊断氨基酸；半乳糖、葡萄糖、马杜糖和核糖作为全细胞糖。检测到的主要甲基萘醌为MK-9(H ₆ )、MK-9(H ₈ )，极性脂质为二磷脂酰甘油、磷脂酰肌醇、糖磷脂和未知磷脂和脂质。其基因组大小约为 10.2 Mb，G+C 含量为 71.6%。菌株 H3C3 ^{T 的}进一步基因组分析表明，其在编码各种化学结构的新型生物合成基因簇方面具有很高的潜力。根据表型和系统发育分析，菌株 H3C3 ^T代表了Actinomadura属的一个新物种，为此Actinomadura rubrisoli sp。十一月 被提出，它对生物制药行业有价值的新型生物合成代谢物具有很高的前景。