Abstract

The creation and use of rust-resistant varieties is the most eco-friendly and efficient method of protecting wheat and triticale crops. For successful selection of spring triticale for rust resistance, it is necessary to have genetic material with efficient genes. In order to identify the carriers of leaf and stem rust-resistant genes, a collection of spring triticale (86 samples) was studied using molecular markers and phytopathology methods. On an artificial infectious background, 81% of collection samples of spring triticale demonstrated a high resistance (0R) to the stem rust population. Nineteen samples that demonstrated a resistance (0–5% R) to the leaf rust population were isolated. Identification of the collection using DNA markers allowed for isolating the samples with Sr2 (19 samples), Sr22 (nine samples), and Lr28 (14 samples) genes. Among the collection, no carriers of Lr9 and Lr35/Sr39 genes were found. The samples with the efficient genes Sr2 and Sr22 were included in crossings to create stem rust-resistant domestic varieties.

中文翻译：

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使用等位基因特异性标记研究春季小黑麦对叶和茎锈病的抗性

摘要

抗锈品种的创造和使用是保护小麦和黑小麦作物的最环保，最有效的方法。为了成功地选择春季小黑麦的耐锈性，必须具有具有有效基因的遗传物质。为了鉴定叶和茎抗锈基因的载体，使用分子标记和植物病理学方法研究了一组春季小黑麦（86个样品）。在人工感染的背景下，春季小黑麦的采集样品中有81％对茎锈病种群表现出高抗性（0R）。分离出19个对叶锈病菌具有抗性（0-5％R）的样品。使用允许使用Sr2分离样品（19个样品）的DNA标记物鉴定馆藏，Sr22（9个样本）和Lr28（14个样本）基因。在收集物中，没有发现Lr9和Lr35 / Sr39基因的载体。具有有效基因Sr2和Sr22的样品被包括在杂交中，以创造出抗茎锈的国内品种。