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Studying a Spring Triticale Collection for Resistance to Leaf and Stem Rusts using Allele-Specific Markers

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Abstract

The creation and use of rust-resistant varieties is the most eco-friendly and efficient method of protecting wheat and triticale crops. For successful selection of spring triticale for rust resistance, it is necessary to have genetic material with efficient genes. In order to identify the carriers of leaf and stem rust-resistant genes, a collection of spring triticale (86 samples) was studied using molecular markers and phytopathology methods. On an artificial infectious background, 81% of collection samples of spring triticale demonstrated a high resistance (0R) to the stem rust population. Nineteen samples that demonstrated a resistance (0–5% R) to the leaf rust population were isolated. Identification of the collection using DNA markers allowed for isolating the samples with Sr2 (19 samples), Sr22 (nine samples), and Lr28 (14 samples) genes. Among the collection, no carriers of Lr9 and Lr35/Sr39 genes were found. The samples with the efficient genes Sr2 and Sr22 were included in crossings to create stem rust-resistant domestic varieties.

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Funding

The work was supported by the Committee of Science of the Ministry of Education and Science of the Republic of Kazakhstan by the budgetary program 217 “Development of Science,” subprogram 102 “Grant Funding for Research,” project no. AP05132430 “Introduction of DNA Markers and Androgenic Technology in Selection of Spring Triticale.”

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Authors and Affiliations

  1. Kazakh Research Institute of Agriculture and Plant Growing, 040909, Almalybak village, Almaty oblast, Republic of Kazakhstan

    R. S. Yerzhebayeva, T. A. Bazylova, D. I. Babissekova, A. A. Amangeldiyeva, D. G. Tajibayev & A. Ydyrys

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  1. R. S. Yerzhebayeva
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  2. T. A. Bazylova
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  3. D. I. Babissekova
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Correspondence to R. S. Yerzhebayeva.

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The authors declare that they have no conflict of interest. No preclinical and clinical studies involving human participants and animals were conducted.

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Translated by A. Barkhash

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Yerzhebayeva, R.S., Bazylova, T.A., Babissekova, D.I. et al. Studying a Spring Triticale Collection for Resistance to Leaf and Stem Rusts using Allele-Specific Markers. Cytol. Genet. 54, 546–554 (2020). https://doi.org/10.3103/S0095452720060043

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