In the present study, a co-culture of yeast (Lipomyces starkeyi) and bacterium (Bacillus cereus) was used to optimize lipid accumulation capability and simultaneous treatment of wastewater using palm oil mill effluent (POME) as a carbon source. The influence of process parameters (i.e., inoculum composition, pH, temperature, and time) on the lipid accumulation and the chemical oxygen demand (COD) removal were optimized using design of experiments (DoE) as a statistical tool. The DoE results suggested that the maximum lipid accumulation of 2.95 g/L and COD removal efficiency of 86.54% could be obtained while the inoculum composition, pH, temperature, and incubation time were 50:50, 6.50, 32.5 °C, and 90 h, respectively. The predicted results were very close to the experimental results (< 5% deviation); hence, the proposed model could be useful to predict the lipid accumulation and COD removal performance of a yeast and bacteria co-culture.

在本研究中，酵母（Lipomyces starkeyi）和细菌（蜡状芽孢杆菌）的共培养）用于优化脂质积累能力，并使用棕榈油厂出水（POME）作为碳源同时处理废水。使用实验设计（DoE）作为统计工具，可以优化工艺参数（即接种物组成，pH，温度和时间）对脂质积累和化学需氧量（COD）去除的影响。DoE结果表明，当接种物组成，pH，温度和孵育时间为50：50、6.50、32.5°C和90 h时，最大脂质积累为2.95 g / L，COD去除率为86.54％。 ， 分别。预测结果与实验结果非常接近（偏差小于5％）；因此，提出的模型可用于预测酵母和细菌共培养的脂质积累和COD去除性能。