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Yeast and bacteria co-culture-based lipid production through bioremediation of palm oil mill effluent: a statistical optimization

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Abstract

In the present study, a co-culture of yeast (Lipomyces starkeyi) and bacterium (Bacillus cereus) was used to optimize lipid accumulation capability and simultaneous treatment of wastewater using palm oil mill effluent (POME) as a carbon source. The influence of process parameters (i.e., inoculum composition, pH, temperature, and time) on the lipid accumulation and the chemical oxygen demand (COD) removal were optimized using design of experiments (DoE) as a statistical tool. The DoE results suggested that the maximum lipid accumulation of 2.95 g/L and COD removal efficiency of 86.54% could be obtained while the inoculum composition, pH, temperature, and incubation time were 50:50, 6.50, 32.5 °C, and 90 h, respectively. The predicted results were very close to the experimental results (< 5% deviation); hence, the proposed model could be useful to predict the lipid accumulation and COD removal performance of a yeast and bacteria co-culture.

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Funding

This study was financially supported by the Ministry of Higher Education (MOHE), Malaysia, through Universiti Malaysia Pahang (FRGS RDU 160150 and PRGS 180317).

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Authors and Affiliations

  1. Faculty of Chemical and Process Engineering Technology, College of Engineering Technology, Universiti Malaysia Pahang, 26300, Gambang, Pahang, Malaysia

    Ahasanul Karim & Che Ku Mohammad Faizal

  2. Laboratory for Quantum Semiconductors and Photon-based BioNanotechnology, Department of Electrical and Computer Engineering, Faculty of Engineering, Université de Sherbrooke, Sherbrooke, Québec, J1K 2R1, Canada

    M. Amirul Islam

  3. Faculty of Civil Engineering Technology, College of Engineering Technology, Universiti Malaysia Pahang, 26300, Gambang, Pahang, Malaysia

    Puranjan Mishra

  4. Faculty of Computing, Universiti Malaysia Pahang, 26600, Pekan, Pahang, Malaysia

    Abu Jafar Md Muzahid

  5. Department of Chemical Engineering and Polymer Science, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh

    Abu Yousuf

  6. Department of Chemical Engineering, College of Engineering, Universiti Malaysia Pahang, 26300, Gambang, Pahang, Malaysia

    Md. Maksudur Rahman Khan

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  1. Ahasanul Karim
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Correspondence to Che Ku Mohammad Faizal.

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Karim, A., Islam, M.A., Mishra, P. et al. Yeast and bacteria co-culture-based lipid production through bioremediation of palm oil mill effluent: a statistical optimization. Biomass Conv. Bioref. 13, 2947–2958 (2023). https://doi.org/10.1007/s13399-021-01275-6

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  • DOI: https://doi.org/10.1007/s13399-021-01275-6

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