A combination of light microscopy and image processing was applied to investigate morphology of label-free primary-melanocytes and melanoma cells. A novel methodological approach based on morphology of nuclear body was used to find those single cells, which were at the same phase of cell cycle. The area and perimeter of melanocytes and melanoma cells were quantified. We found that there was a significant difference between area and perimeter of adendritic-shaped melanocytes with melanoma cells and the reason(s) of this finding was speculated. Finally, a theoretical model based on losing dendrites was proposed, which was in agreement with our experimental data.

中文翻译：

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无标记人黑色素细胞和黑色素瘤细胞的形态测量和建模

应用光学显微镜和图像处理的组合来研究无标记原代黑色素细胞和黑色素瘤细胞的形态。一种新的基于核体形态的方法学方法被用来寻找那些处于细胞周期同一阶段的单细胞。量化黑素细胞和黑素瘤细胞的面积和周长。我们发现腺突状黑色素细胞与黑色素瘤细胞的面积和周长存在显着差异，并推测了这一发现的原因。最后，提出了基于失去枝晶的理论模型，与我们的实验数据一致。