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Isolation and optimization of a novel thraustochytrid strain for DHA rich and astaxanthin comprising biomass as aquafeed supplement
3 Biotech ( IF 2.6 ) Pub Date : 2021-01-13 , DOI: 10.1007/s13205-020-02616-4
Pratik R Pawar 1 , Sneha Velani 1 , Sujata Kumari 1 , Arvind M Lali 2 , Gunjan Prakash 1
Affiliation  

A marine organism, belonging to the Thraustochytrids family was isolated from mangroves of Mumbai, India. The isolated strain was identified as Aurantiochytrium limacinum by internal transcribed spacer sequence analysis. Optimization of process parameters yielded 14.47 g/L dry cell weight containing 55–58% oil in 3.5 days’ cultivation on glucose, yeast extract, and peptone in the bioreactor. Docosahexaenoic acid (DHA) was found to be the dominant long-chain polyunsaturated fatty acid, accounting for 32–35% of total fatty acid content. The process parameter was tweaked to simultaneously synthesize astaxanthin along with DHA. The concurrent synthesis of DHA and astaxanthin-containing biomass establishes the isolated strain as a perfect choice for aquafeed.

Accession number: NCBI accession number MN046792.



中文翻译:

一种新型破囊壶菌菌株的分离和优化,用于富含 DHA 和虾青素的生物质作为水产饲料补充剂

从印度孟买的红树林中分离出一种属于破囊壶菌科的海洋生物。分离株 经内转录间隔序列分析鉴定为Aurantiochytrium limacinum  在生物反应器中对葡萄糖、酵母提取物和蛋白胨进行 3.5 天的培养后,工艺参数的优化产生了 14.47 g/L 的干细胞重量,其中含有 55-58% 的油。发现二十二碳六烯酸 (DHA) 是主要的长链多不饱和脂肪酸,占总脂肪酸含量的 32-35%。调整工艺参数以同时合成虾青素和 DHA。同时合成 DHA 和含有虾青素的生物质使分离菌株成为水产饲料的完美选择。

登录号:NCBI 登录号 MN046792。

更新日期:2021-01-13
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