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Isolation and optimization of a novel thraustochytrid strain for DHA rich and astaxanthin comprising biomass as aquafeed supplement

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Abstract

A marine organism, belonging to the Thraustochytrids family was isolated from mangroves of Mumbai, India. The isolated strain was identified as Aurantiochytrium limacinum by internal transcribed spacer sequence analysis. Optimization of process parameters yielded 14.47 g/L dry cell weight containing 55–58% oil in 3.5 days’ cultivation on glucose, yeast extract, and peptone in the bioreactor. Docosahexaenoic acid (DHA) was found to be the dominant long-chain polyunsaturated fatty acid, accounting for 32–35% of total fatty acid content. The process parameter was tweaked to simultaneously synthesize astaxanthin along with DHA. The concurrent synthesis of DHA and astaxanthin-containing biomass establishes the isolated strain as a perfect choice for aquafeed.

Accession number: NCBI accession number MN046792.

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Authors and Affiliations

  1. DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, India

    Pratik R. Pawar, Sneha Velani, Sujata Kumari & Gunjan Prakash

  2. Department of Chemical Engineering, Institute of Chemical Technology, Mumbai, India

    Arvind M. Lali

Authors
  1. Pratik R. Pawar
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  2. Sneha Velani
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  3. Sujata Kumari
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  4. Arvind M. Lali
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  5. Gunjan Prakash
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Contributions

Experiments were planned by GP and AML. Isolation work was executed by SV. SK planned and executed the strain identification studies. PRP did the growth optimization experiments. Data were analysed by PRP, SK, AML and GP. The manuscript is written by PRP and GP. Funding for the work was facilitated by AML.

Corresponding author

Correspondence to Gunjan Prakash.

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Pawar, P.R., Velani, S., Kumari, S. et al. Isolation and optimization of a novel thraustochytrid strain for DHA rich and astaxanthin comprising biomass as aquafeed supplement. 3 Biotech 11, 71 (2021). https://doi.org/10.1007/s13205-020-02616-4

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