There is growing concern about developing treatment technologies for the hazardous Polycyclic Aromatic Hydrocarbons (PAHs), because the rising levels of these compounds in the environment by human activities. The application of laccases has been evaluated as one of the most promising treatments. Thus, laccase immobilization on polyurethane foam (PUF)—low-cost material—was evaluated for bioremediation in batch mode of simulated groundwater using a combination of 16 polycyclic aromatic hydrocarbons as model pollutants. Conditions closer to a real contaminated site (non-optimal) were considered on our experimental design, leading to the formation of new degradation intermediaries, even more degraded than the usual ones. The bioremediation of PAH (mg L⁻¹) using immobilized laccase on PUF reached 92.35% of removal for anthracene (Ant) and 97% for benzo(a)pyrene (BaP). After the treatment, the biodegradation products were identified as diisooctyl phthalate and tetradecane. The biodegradation mechanism was proposed, where PAHs oxidation processes and aromatic ring fission led to quinone and diethyl phthalate formation. Then, through the latter processes besides, polymerization and methylation, lead to the identified biodegradation product formation. The immobilized enzyme improvement in the removal yield of 8 of the other 14 PAHs tested in μg L⁻¹, compared to the free counterpart. Laccase immobilized on PUF achieved final anthracene concentration of 0.95 mg L⁻¹, up to 38 μg L⁻¹ of chrysene (77% removal) and 98 μg L⁻¹ of pyrene (32% removal), under the intervention limits of environmental protection policies. Thus, laccase immobilized on PUF for use in bioreactors can be considered a potential approach for PAHs bioremediation for an ex-situ treatment.

人们越来越关注开发有害多环芳烃（PAH）的处理技术，因为人类活动导致环境中这些化合物的含量不断上升。漆酶的应用已被评估为最有前途的治疗方法之一。因此，使用16种多环芳烃作为模型污染物，以模拟地下水的批处理方式评估了漆酶固定在低成本材料聚氨酯泡沫（PUF）上的生物修复作用。在我们的实验设计中考虑了更接近实际污染地点（非最佳）的条件，这导致了新的降解中间体的形成，甚至比通常的降解中间体还要降解。PAH的生物修复（mg L ^-1）在PUF上使用固定的漆酶可去除92.35％的蒽（Ant）和97％的苯并（a）re（BaP）。处理后，生物降解产物被鉴定为邻苯二甲酸二异辛酯和十四烷。提出了生物降解机理，其中PAHs的氧化过程和芳环裂变导致醌和邻苯二甲酸二乙酯的形成。然后，除了聚合和甲基化之外，还通过后面的过程导致确定的生物降解产物的形成。与游离的对等物相比，固定化酶对其他14种PAH中的8种的去除率提高了，以微克L ^-1计。固定在PUF上的漆酶的最终蒽浓度达到0.95 mg L ^-1，最高达到38μgL ^-1在环境保护政策的干预范围内，苯的去除率（77％）和98μgL ^-1的（（32％去除率）。因此，固定在PUF上用于生物反应器的漆酶可以被认为是PAHs生物修复用于异位治疗的潜在方法。