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Application of Immobilized Laccase on Polyurethane Foam for Ex-Situ Polycyclic Aromatic Hydrocarbons Bioremediation

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Abstract

There is growing concern about developing treatment technologies for the hazardous Polycyclic Aromatic Hydrocarbons (PAHs), because the rising levels of these compounds in the environment by human activities. The application of laccases has been evaluated as one of the most promising treatments. Thus, laccase immobilization on polyurethane foam (PUF)—low-cost material—was evaluated for bioremediation in batch mode of simulated groundwater using a combination of 16 polycyclic aromatic hydrocarbons as model pollutants. Conditions closer to a real contaminated site (non-optimal) were considered on our experimental design, leading to the formation of new degradation intermediaries, even more degraded than the usual ones. The bioremediation of PAH (mg L−1) using immobilized laccase on PUF reached 92.35% of removal for anthracene (Ant) and 97% for benzo(a)pyrene (BaP). After the treatment, the biodegradation products were identified as diisooctyl phthalate and tetradecane. The biodegradation mechanism was proposed, where PAHs oxidation processes and aromatic ring fission led to quinone and diethyl phthalate formation. Then, through the latter processes besides, polymerization and methylation, lead to the identified biodegradation product formation. The immobilized enzyme improvement in the removal yield of 8 of the other 14 PAHs tested in μg L−1, compared to the free counterpart. Laccase immobilized on PUF achieved final anthracene concentration of 0.95 mg L−1, up to 38 μg L−1 of chrysene (77% removal) and 98 μg L−1 of pyrene (32% removal), under the intervention limits of environmental protection policies. Thus, laccase immobilized on PUF for use in bioreactors can be considered a potential approach for PAHs bioremediation for an ex-situ treatment.

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Acknowledgements

The authors wish to thank the Brazilian Agency CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for the financial support and to UNIVILLE-Universidade da Região de Joinville, where the experiments were carried out.

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Authors and Affiliations

  1. Department of Chemical and Food Engineering, Federal University of Santa Catarina, Campus Universitário Reitor João David Ferreira Lima, Florianópolis, Santa Catarina, 88040-900, Brazil

    Brayam Luiz Batista Perini, Naionara Ariete Daronch, Cristiano José de Andrade & Débora de Oliveira

  2. Department of Chemistry, Center of Technological Sciences, Santa Catarina State University, N. 200 Paulo Malschitzki Street, Joinville, SC, 89219-710, Brazil

    Rodrigo Luiz Bitencourt

  3. Inova Laboratory and Engineering, N. 143 Jose Mattei Street, Joinville, SC, 89226-538, Brazil

    Rodrigo Luiz Bitencourt

  4. Departament of Chemical Engineering, University of Joinville Region, N. 10 Paulo Malschitzki Street, Joinville, SC, 89219-710, Brazil

    Andréa Lima dos Santos Schneider

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  1. Brayam Luiz Batista Perini
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  3. Rodrigo Luiz Bitencourt
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BP, DO and AS conceived and designed research. BP and ND conducted experiments. RB contributed analytical tools. BP, CA and DO wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Débora de Oliveira.

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Perini, B.L.B., Daronch, N.A., Bitencourt, R.L. et al. Application of Immobilized Laccase on Polyurethane Foam for Ex-Situ Polycyclic Aromatic Hydrocarbons Bioremediation. J Polym Environ 29, 2200–2213 (2021). https://doi.org/10.1007/s10924-020-02035-9

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