Purslane (Portulaca oleracea L.), a valuable medicinal herb, is used as a source of pharmaceutical components such as flavonoids, alkaloids, fatty acids, terpenoids, and sterols. Regeneration of transgenic purslane plantlets from transformed cells is a time-consuming procedure and needs hard work. In this study, in-planta transformation of purslane, using sonication and vacuum infiltration, is reported. The purslane seeds were infected through Agrobacterium tumefaciens strain LBA4404 having the uidA gene on pBI121 vector. Effective selection of transformants was performed by supplementing MS media with 250 mg l⁻¹ kanamycin. Several factors affecting the in-planta procedure including pre-culture duration, acetosyringone dose, sonication, and vacuum infiltration duration were investigated. The results demonstrated that the highest number of GUS-positive purslane plantlets was obtained when the pre-cultured seeds were sonicated and vacuum-infiltered for 2 min in agro-bacterial cell suspension, and then co-cultivated in MS media having 100 µM acetosyringone. The integration and expression of uidA gene in transgenic purslane was successfully corroborated by southern blot and GUS histochemical analyses.

马齿ane（Portulaca oleracea L.）是一种有价值的药用植物，被用作黄酮类，生物碱，脂肪酸，萜类和固醇等药物成分的来源。从转化细胞再生转基因马齿plant小植株是一个耗时的过程，需要艰苦的工作。在这项研究中，报道了利用超声处理和真空渗透进行马齿sl在植物体内的转化。通过在pBI121载体上具有uid A基因的根癌土壤杆菌菌株LBA4404感染马齿seeds种子。通过向MS培养基补充250 mg l ^-1卡那霉素来进行转化子的有效选择。影响种植体内的几个因素研究了包括培养前持续时间，乙酰丁香酮剂量，超声处理和真空渗透持续时间在内的程序。结果表明，将预培养的种子在农杆菌细胞悬液中超声处理并真空过滤2分钟，然后在含有100 µM乙酰丁香酮的MS培养基中共培养，可获得最高数量的GUS阳性马齿plant小植株。Southern印迹和GUS组织化学分析成功证实了uid A基因在转基因马齿sl中的整合和表达。