Ranunculus wallichianus is a rare medicinal plant endemic to Western Ghats, India. Nodal explants were inoculated on MS medium with 1.0 to 5.0 mg L⁻¹ of 6-benzyladenine (BA), kinetin (KIN), or thidiazuron (TDZ) resulting in a low percentage of shoot regeneration. Coconut water (CW) was added to MS medium containing the above mentioned cytokinins to promote in vitro plantlet growth. Multiple shoots were regenerated on half-strength Murashige and Skoog (MS) medium containing 1.0 to 5.0 mg L⁻¹ BA, KIN, or TDZ in combination with 5%, 10%, and 20% CW. The highest percentage of shoot multiplication was observed from cultures incubated on half-strength MS medium supplemented with 3.0 mg L⁻¹ TDZ in combination with 10% CW. The cut ends of well-elongated shoots were transferred to medium containing 0.5 to 3.0 mg L⁻¹ indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) to induce in vitro root induction. Half-strength MS medium with 2.5 mg L⁻¹ IBA and 250 mg L⁻¹ activated charcoal shows high frequency of root formation. The well-rooted plantlets were transferred to pots for hardening with survival rate of 93% after 30 d. The present work indicates the addition of CW with the cytokinins in half-strength MS medium is suitable for rapid micropropagation of R. wallichianus.

毛an属Wallichianus是印度西高止山脉特有的稀有药用植物。将结节外植体接种在含有1.0至5.0 mg L ^-1的6-苄基腺嘌呤（BA），激动素（KIN）或噻二唑酮（TDZ）的MS培养基上，导致芽再生的百分比较低。椰子水（CW）中的溶液加入到含有上述细胞分裂素促进MS培养基中体外植株的生长。在含有1.0至5.0 mg L ^-1 BA，KIN或TDZ以及5％，10％和20％CW的半强度Murashige和Skoog（MS）培养基上再生多个芽。从在补充了3.0 mg L ^-1的半强度MS培养基上培养的培养物中观察到最高的芽繁殖百分比TDZ与10％的CW结合使用。将充分伸长的芽的切端转移至含有0.5至3.0mg L ^-1吲哚-3-乙酸（IAA）或吲哚-3-丁酸（IBA）的培养基中以诱导体外根诱导。具有2.5 mg L ^-1 IBA和250 mg L ^-1活性炭的半强度MS培养基显示出很高的根形成频率。将根深蒂固的小苗转移到盆中进行硬化，30天后成活率为93％。目前的工作表明在半强度MS培养基中添加细胞分裂素与CW的方法适合于Wallichianus的快速微繁殖。