Skip to main content
SpringerLink
Log in

Effect of coconut water and cytokinins on rapid micropropagation of Ranunculus wallichianus Wight & Arnn—a rare and endemic medicinal plant of the Western Ghats, India

  • Medicinal Plants
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

Ranunculus wallichianus is a rare medicinal plant endemic to Western Ghats, India. Nodal explants were inoculated on MS medium with 1.0 to 5.0 mg L−1 of 6-benzyladenine (BA), kinetin (KIN), or thidiazuron (TDZ) resulting in a low percentage of shoot regeneration. Coconut water (CW) was added to MS medium containing the above mentioned cytokinins to promote in vitro plantlet growth. Multiple shoots were regenerated on half-strength Murashige and Skoog (MS) medium containing 1.0 to 5.0 mg L−1 BA, KIN, or TDZ in combination with 5%, 10%, and 20% CW. The highest percentage of shoot multiplication was observed from cultures incubated on half-strength MS medium supplemented with 3.0 mg L−1 TDZ in combination with 10% CW. The cut ends of well-elongated shoots were transferred to medium containing 0.5 to 3.0 mg L−1 indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) to induce in vitro root induction. Half-strength MS medium with 2.5 mg L−1 IBA and 250 mg L−1 activated charcoal shows high frequency of root formation. The well-rooted plantlets were transferred to pots for hardening with survival rate of 93% after 30 d. The present work indicates the addition of CW with the cytokinins in half-strength MS medium is suitable for rapid micropropagation of R. wallichianus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Agampodi VA, Jayawardena B (2009) Effects of coconut (Cocos nucifera L.) water extracts on adventitious root development in vegetative propagation of Dracaena purplecompacta L. Acta Plant Physiol 31:279–284

    Article  Google Scholar 

  • Akkol EK, Süntar I, Erdoğan TF, Keleş H, Gonenç TM, Kıvçak B (2012) Wound healing and anti-inflammatory properties of Ranunculus pedatus and Ranunculus constantinapolitanus: comparative study. J Ethnopharmacol 139:478–484

    Article  PubMed  Google Scholar 

  • Al-Khayri JM (2010) Somatic embryogenesis of date palm (Phoenix dactylifera L.) improved by coconut water. Biotechnol 9:477–484

    Article  Google Scholar 

  • Aslam MS, Choudhary BA, Uzair M, Ijaz AS (2012) The genus Ranunculus: a phytochemical and ethnopharmacological review. Int J Pharm Pharm Sci 4:1–8

    Google Scholar 

  • Baque MA, Shin YK, Elshmari T, Lee EJ, Peak KY (2011) Effect of light quality, sucrose and coconut water concentration on the microropagation of Calanthe hybrids (Bukduseong ˟ Hyesung and Chunkwang ˟ Hyesung). Aust J Crop Sci 5:1247–1254

    CAS  Google Scholar 

  • Beruto M, Debergh P (1992) Somatic embryogenesis in Ranunculus asiaticus L. hybr. Thalamus cultivated in vitro. Plant Cell Tiss Org Cult 29:161–165

    Article  CAS  Google Scholar 

  • Beruto M, Debergh P (2004) Micropropagationof Ranunculus asiaticus: a review and perspectives. Plant Cell Tiss Org Cult 77:221–230

    Article  Google Scholar 

  • Beruto M, Curir P, Debergh P (1996) Callus growth and somatic embryogenesis in thalamus tissue of Ranunculus asiaticus L. Cultivated in vitro: cytokinin effect and phenol metabolism. In Vitro Cell Dev Biol - Plant 32:154–160

    Article  CAS  Google Scholar 

  • Beruto M, Curir P, Debergh P (1999) Influence of agar on in vitro cultures: II. Biological performance of Ranunculus on media solidified with three different agar brands. In Vitro Cell Dev Biol - Plant 35:94–101

    Article  CAS  Google Scholar 

  • Bicknell RA, Braun RH, Evans AC, Morgan ER (1996) Tissue culture of Ranunculus lyallii Hook. f. New Zealand. J Crop Hort Sci 24:303–306

    Article  Google Scholar 

  • Cao BJ, Meng QY, Ji N (1992) Analgesic and anti-inflammatory effects of Ranunculus japonicus extract. Planta Med 58:496–498

    Article  CAS  PubMed  Google Scholar 

  • Cheruvathur MK, Kumar GK, Thomas TD (2013) Somatic embryogenesis and synthetic seed production in Rhinacanthus nasutus (L.) Kurz. Plant Cell Tiss Org Cult 113:63–71

    Article  CAS  Google Scholar 

  • Choudhary D, Rai MK, Shekhawat NS, Kataria V (2020) In vitro propagation of Farsetia macrantha Blatt. & Hallb.: an endemic and threatened plant of Indian Thar Desert. Plant Cell Tiss Org Cult 142:519–526

    Article  CAS  Google Scholar 

  • Dubey RC (1999) A text book of biotechnology. S. Chand & Co. P Ltd, New Delhi

    Google Scholar 

  • Faisal M, Ahmad N, Anis M (2005) Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tiss Org Cult 80:119–127

    Article  Google Scholar 

  • Fatima N, Ahmad N, Ahmad I, Anis M (2015) Interactive effects of growth regulators, carbon sources, pH on plant regeneration and assessment of genetic fidelity using single primer amplification reaction (SPARS) techniques in Withania somnifera L. Appl Biochem Biotechnol 177:118–136

    Article  CAS  PubMed  Google Scholar 

  • Fay MG (1992) Conservation of rare and endangered plants using in in vitro methods. In Vitro Cell Dev Biol - Plant 28:1–4

    Article  Google Scholar 

  • Feyissa T, Welandr M, Negash L (2005) Micropropagation of Hagenia abyssinica (Bruce) J. F. Gmel: a multipurpose tree. Plant Cell Tiss Org Cult 80:119–127

    Article  Google Scholar 

  • George E (1993) Plant propagation by tissue culture, 2nd edn. Exegetics Ltd, London, p 457

    Google Scholar 

  • Gomez KA, Gomez AA (1976) Statistical procedures for agricultural research with emphasis on Rice, Los Banos. Philippines Intl Rice Res 50:155–158

    Google Scholar 

  • Guo B, Abbasi BH, Zeb A, Xu LL, Wei YH (2011) Thidiazuron: a multi-dimensional plant growth regulator. Afr J Biotechnol 10:8984–9000

    Article  CAS  Google Scholar 

  • Guo B, Stiles AR, Liu CZ (2012) Thidiazuron enhances shoot organogenesis from leaf explants of Saussurea involucrata Kar et. Kir. In Vitro Cell Dev Biol - Plant 48:609–612

    Article  CAS  Google Scholar 

  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss Org Cult 33:105–119

    Article  CAS  Google Scholar 

  • Iqbal H, Sher Z, Khan ZU (2011) Medicinal plants from salt range Pind Dadan Khan, district Jhelum, Punjab. Pak J Med Plants Res 5:2157–2168

    Google Scholar 

  • Kaya G, Ünver Somer N, Konyalioǧlu S, Yalçin HT, Karabay Yavaşoǧlu N, Sarikaya B, Önür M (2010) Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. Turk J Biol 34:139–146

    CAS  Google Scholar 

  • Kumar KK (2004) Taxonomic and ecological studies on the shola forests of Kerala. Ph. D Thesis. Kerala Forest Research Institute Thrissur, University of Calicut

  • Loc BH, Duc DT, Kwon TH (2005) Micropropagation of Zedoary (Curcuma zeloaria, Roscoe)-valuable medicinal plants. Plant Cell Tiss Org Cult 81:119–122

    Article  CAS  Google Scholar 

  • Mares D (1987) Antimicrobiol activity of Protoanemonin, a lactose from Ranunculaceous plant. Mycopathologia 98:133–140

    Article  CAS  PubMed  Google Scholar 

  • Mathew KM (1996) Flora of the Palni Hills. The Rapinat Herbarium, Tiruchirapalli

    Google Scholar 

  • Meynet J, Duclos A (1990) Culture in vitro de la renoncule des fleuristes (Ranunculus asiaticus L.). II. Production de plantes par cultured’anthères in vitro. Agronomie 10:213–218

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nasib A, Ali K, Khan S (2008) An optimized and improved method for the in vitro propagation of kiwifruit (Actinidia deliciosa) using coconut water. Pak J Bot 40:2355–2360

    CAS  Google Scholar 

  • Pathnaik SK, Chand PK (1996) In vitro propagation of the medicinal herbs Ocimum americanum. Syn. Ocimum sims hoary basil and Ocimum sanctum (Holly Basil). Plant Cell Rep 15:846–860

    Article  Google Scholar 

  • Pugliesi C, Rabaglio M, Cecconi F, Baroncelli S (1992) Plant regeneration from tissue cultures of Persian buttercup (Ranunculus asiaticus L.). Plant Cell Tiss Org Cult 28:125–128

    Article  CAS  Google Scholar 

  • Roy J, Banerjee N (2002) Rhizome and shoot development during in vitro propagation of Geordum densiflorum(Lam.). Schltr Sci Hortic 94:181–192

    Article  CAS  Google Scholar 

  • Siddique I, Anis M (2007) In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia (Vahl.): a medicinal plant. Acta Physiol Plant 29:233–328

    Article  CAS  Google Scholar 

  • Sujatha G, Ranjitha Kumari BD (2007) High frequency shoot multiplication in Artemisia vulgaris L. using thidiazuron. Plant Biotechnol Rep 1:149–154

    Article  Google Scholar 

  • Tamura M (1993) Ranunculaceae. In: Kubitzki K, Rohwer JG, Bittrich V (eds) The families and genera of vascular plants, vol. 2, flowering plants: Dicotyledons, Magnoliid, Hamamelid, and Caryophyllid families. Springer, Berlin, pp 563–583

    Google Scholar 

  • Tamura M (1995) Angiospermae. Ordnung Ranunculales. Fam. Ranunculaceae. II. Systematic Part. In: Hiepko P (ed) Die Natürliche Pflanzenfamilien, vol 17aIV, 2nd edn. Duncker Humblot, Berlin, pp 223–519

    Google Scholar 

  • Tan BC, Chin CF, Alderson P (2011) An improved plant regeneration of Vanilla planifolia Andrews. Plant Tiss Cult Biotechnol 21:27–33

    Article  Google Scholar 

  • Tiwari V, Tiwari KN, Singh BD (2001) Comparative studies cytokinins on in vitro propagation of Bacopa monniera. Plant Cell Tiss Org Cult 66:9–16

    Article  CAS  Google Scholar 

  • Vaidya BN, Jackson CL, Perry ZD, Dhekney SA, Joshee N (2016) Agrobacterium-mediated transformation of thin cell layer explants of Scutellaria ocmulgee small: a rare plant with anti-tumor properties. Plant Cell Tiss Org Cult 127:57–69

    Article  CAS  Google Scholar 

  • Yin CP, Fan LC, Zhang LD, Lin ZC, He JW, Liu MN, Wang YQ (2008) The inhibiting effect of extracts in Radix Ranunculi Ternati on the growth of human breast cancer cells in vitro. Chin J Hosp Pharm 28:93–96

    CAS  Google Scholar 

Download references

Acknowledgments

Authors gratefully acknowledge the management of St. Joseph’s college, Tiruchirappalli, India, for providing the laboratory facilities.

Funding

The authors also acknowledge the University Grant Commission (UGC)–South Eastern Regional Office (SERO), Hyderabad (MRP-5973/15-UGC SERO), India, for financial support.

Author information

Authors and Affiliations

  1. Gnanarethinam Centre for In vitro Conservation (GCIC), Department of Botany, St. Joseph’s College, Affiliated to Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620002, India

    P. Srinivasan, H. David Raja & R. Tamilvanan

Authors
  1. P. Srinivasan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. David Raja
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Tamilvanan
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

PS and RT carry out the experiments and analyze the experimental data. PS prepared the manuscript. HDR gave the outline of the experimental design and edited the final version of the manuscript.

Corresponding author

Correspondence to P. Srinivasan.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Editor: Prakash Lakshmanan

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Srinivasan, P., Raja, H.D. & Tamilvanan, R. Effect of coconut water and cytokinins on rapid micropropagation of Ranunculus wallichianus Wight & Arnn—a rare and endemic medicinal plant of the Western Ghats, India. In Vitro Cell.Dev.Biol.-Plant 57, 365–371 (2021). https://doi.org/10.1007/s11627-020-10137-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-020-10137-1

Keywords

Search

Navigation