Bone marrow-derived mesenchymal stem cells (BMSCs) have been considered a promising therapeutic approach to cardiovascular disease. This study intends to compare the effect of BMSCs through a standard active cardiac support device (ASD) and intravenous injection on global myocardial injury induced by isoproterenol. BMSCs were cultured in vitro, and the transplanted cells were labeled with a fluorescent dye CM-Dil. Isoproterenol (ISO) was injected into the rats; 2 weeks later, the labeled cells were transplanted into ISO-induced heart-jury rats through the tail vein or ASD device for 5 days. The rats were sacrificed on the first day, the third day, and the fifth day after transplantation to observe the distribution of cells in the myocardium by fluorescence microscopy. The hemodynamic indexes of the left ventricle were measured before sacrificing. H&E staining and Masson’s trichrome staining were used to evaluate the cardiac histopathology. In the ASD groups, after 3 days of transplantation, there were a large number of BMSCs on the epicardial surface, and after 5 days of transplantation, BMSCs were widely distributed in the ventricular muscle. But in the intravenous injection group, there were no labeled-BMSCs distributed. In the ASD + BMSCs-three days treated group and ASD + BMSCs -five days-treated group, left ventricular systolic pressure (LVSP), the maximum rate of left ventricular pressure rise (+dP/dt), the maximum rate of left ventricular pressure decline (-dP/dt) increased compared with model group and intravenous injection group (P < 0.05). By giving BMSCs through ASD device, cells can rapidly and widely distribute in the myocardium and significantly improve heart function.

骨髓间充质干细胞 (BMSCs) 被认为是一种很有前途的心血管疾病治疗方法。本研究旨在比较 BMSCs 通过标准主动心脏支持装置 (ASD) 和静脉注射对异丙肾上腺素诱导的整体心肌损伤的影响。BMSCs体外培养, 移植的细胞用荧光染料 CM-Dil 标记。给大鼠注射异丙肾上腺素（ISO）；2 周后，标记的细胞通过尾静脉或 ASD 装置移植到 ISO 诱导的心脏损伤大鼠中 5 天。移植后第1天、第3天、第5天处死大鼠，荧光显微镜观察心肌细胞分布。牺牲前测量左心室的血流动力学指标。H&E 染色和 Masson 三色染色用于评估心脏组织病理学。ASD组移植3天后心外膜表面有大量BMSCs，移植5天后BMSCs广泛分布于心室肌。但在静脉注射组中，没有标记的 BMSCs 分布。在ASD+BMSCs-3天治疗组和ASD+BMSCs-5天治疗组中，左心室收缩压（LVSP）、左心室压力上升最大速率（+dP/dt）、左心室最大速率与模型组和静脉注射组相比，压力下降（-dP/dt）增加（P  < 0.05)。通过ASD装置给予骨髓间充质干细胞，细胞可以迅速广泛分布在心肌中，显着改善心脏功能。