Abstract

A nano-complex containing fish scale collagen, konjac glucomannan, camellia chrysantha polyphenols and ginsenoside Rb1 (GCPR) have been prepared successfully by gelation method. Infrared spectroscopy (IR), scanning field emission scanning electron microscopy (FESEM), Energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), ultraviolet–visible spectroscopy (UV–Vis) were used to determine characteristics of obtained GCPR nano-complex. The GCPR nano-complex is in spherical shape with the average particle size is 115.4 nm (95.1%) and 593.0 nm (4.1%). Polyphenols and ginsenoside Rb1 could interact with collagen and konjac glucomannan through hydrogen bonding and dipole–dipole interactions, therefore, the drug release from the GCPR nano-complex in pH 2 buffer solution (simulated gastric fluid in the human body) and pH 7.4 buffer solution (simulated intestinal fluid in the human body) could be controlled. In particular, biological activity of the GCPR nano-complex in inhibition of cancer cells, anti-inflammatory and antioxidant also was investigated. The obtained results show that the GCPR nano-complex exhibits a positive effect on inhibition of cancer cells and anti-inflammatory in comparison with polyphenol or ginsenoside Rb1. Besides, the GCPR nano-complex and polyphenol also exhibit non-toxic to normal cells and antioxidation ability. This nano-complex is promising for application in biomedicine.

Graphic Abstract

中文翻译：

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基于鱼鳞胶原蛋白，魔芋葡甘露聚糖，山茶多酚和人参皂苷Rb1的新型生物聚合物纳米复合物的制备，表征及其生物活性

摘要

含有鱼鳞胶原蛋白，魔芋葡甘露聚糖，山茶山ry的纳米复合物通过凝胶法成功制备了多酚和人参皂苷Rb1（GCPR）。红外光谱（IR），扫描场发射扫描电子显微镜（FESEM），能量色散X射线光谱（EDX），动态光散射（DLS），紫外可见光谱（UV-Vis）用于确定所得化合物的特性。 GCPR纳米复合物。GCPR纳米复合物呈球形，平均粒径为115.4 nm（95.1％）和593.0 nm（4.1％）。多酚和人参皂苷Rb1可以通过氢键和偶极-偶极相互作用与胶原蛋白和魔芋葡甘露聚糖相互作用，因此，药物在pH 2缓冲液（模拟的人体胃液）和pH 7.4缓冲液中从GCPR纳米复合物中释放出来。 （人体中的模拟肠液）可以得到控制。特别是，还研究了GCPR纳米复合物在抑制癌细胞，抗炎和抗氧化剂方面的生物学活性。获得的结果表明，与多酚或人参皂苷Rb1相比，GCPR纳米复合物对癌细胞的抑制和抗炎性具有积极的作用。此外，GCPR纳米复合物和多酚对正常细胞也无毒，并具有抗氧化能力。这种纳米复合物有望用于生物医学。GCPR纳米复合物和多酚对正常细胞也无毒，并具有抗氧化能力。这种纳米复合物有望用于生物医学。GCPR纳米复合物和多酚对正常细胞也无毒，并具有抗氧化能力。这种纳米复合物有望用于生物医学。

图形摘要