Sulfur limitation is a frequently exploited method for inducing lipid accumulation in microalgae. However, there is little understanding of the underlying mechanism of this process. Hence, a study was conducted to determine the effects of sulfur limitation on nitrogen and sulfur uptake, lipid production strategies, and photosynthetic physiological characteristics of Scenedesmus acuminatus. A low sulfur concentration (0.076 mM; 0.25S group) decreased nitrate reductase activity and inhibited nitrogen absorption. The highest lipid productivity was obtained in the 0.75S (0.229 mM) group (221.93 mg L⁻¹ day⁻¹), significantly higher than that of the 0.5S (0.153 mM) and 0.25S groups (p < 0.05). Acetyl-coenzyme A carboxylase (ACCase) activity was upregulated in the 0.25S group to promote the accumulation of lipid and fatty acids, and generated the maximum lipid and fatty acid content (47.27% and 43.98% of dry weight, respectively). An important supplementation of energy for lipid accumulation under low-sulfur conditions is to drive a higher proportion of cyclic electron flow. Superoxide dismutase and peroxidase activities and malondialdehyde content increased at lower sulfur concentrations, indicating the elevated antioxidant activity of S. acuminatus.

硫限制是诱导微藻类中脂质积累的一种常用方法。但是，对此过程的基本机制了解甚少。因此，进行了一项研究来确定硫限制对氮和硫吸收，脂质产生策略以及灵芝（Scendesmus acuminatus）光合生理特性的影响。低硫浓度（0.076 mM； 0.25S组）降低了硝酸盐还原酶的活性并抑制了氮的吸收。在0.75S（0.229 mM）组（221.93 mg L ^-1 day ^-1）中获得了最高的脂质生产率，显着高于0.5S（0.153 mM）和0.25S组（p<0.05）。0.25S组的乙酰辅酶A羧化酶（ACCase）活性上调，以促进脂质和脂肪酸的积累，并产生最大的脂质和脂肪酸含量（分别为干重的47.27％和43.98％）。在低硫条件下脂质积累的重要能量补充是驱动更高比例的循环电子流。超氧化物歧化酶和过氧化物酶活性和丙二醛含量增加在较低的硫浓度，表示的升高的抗氧化活性小号。尖锐虫。