Traditional in vitro evaluation criteria of magnesium (Mg)-based stents cannot reflect the degradation process in vivo, due to the interdependence and interference between biodegradable properties and bioenvironment. The current direct and indirect evaluation approaches of in vitro biocompatibility do not have a hydrodynamic environment and vascular biological structure existing in vivo. Herein, we designed a vascular bioreactor to provide an ex vivo culture environment for vessels, which reveals the degradation behavior of Mg–Zn–Mn stent and the effect of its degradation on cells. We reported that rabbit carotid arteries could maintain native morphology and viability in the bioreactor under the best condition within a flow rate of 5.4 mL min⁻¹ and a culture time of one week. With this culture condition, Mg–Zn–Mn stents were implanted into the arteries in the bioreactors and compared with in vivo rabbit models. The arteries maintained cell survival in the bioreactor, but the cell attachment was absent on the stent struts, associated with a fast degradation. Conversely, the stents achieved a rapid and complete endothelialization in vivo for two weeks. This study could provide a correlation and difference of the degradation behavior and cellular response to the degradation of Mg-based stent between ex vivo and in vivo approaches.

由于可生物降解特性与生物环境之间的相互依赖和干扰，传统的镁（Mg）基支架的体外评价标准不能反映体内降解过程。目前体外生物相容性的直接和间接评价方法不存在体内存在的水动力环境和血管生物结构。在这里，我们设计了一个血管生物反应器来提供离体血管的培养环境，揭示了 Mg-Zn-Mn 支架的降解行为及其降解对细胞的影响。我们报告说，兔颈动脉可以在5.4 mL min ^{-1 的}流速和一周的培养时间内在最佳条件下保持生物反应器中的天然形态和活力。在这种培养条件下，将 Mg-Zn-Mn 支架植入生物反应器的动脉中，并与体内兔模型进行比较。动脉在生物反应器中维持细胞存活，但支架支柱上没有细胞附着，这与快速降解有关。相反，支架在体内实现了快速且完全的内皮化两周。该研究可以提供体外和体内方法之间降解行为和细胞对镁基支架降解的反应的相关性和差异。