The effects of platinum (Pt) and gold (Au) and on the soil bacterial community was evaluated in four different Australian soil types (acidic Burn Grounds (BGR), organic matter–rich Fox Lane, high silt/metal Pinpinio (PPN), and alkali Minnipa (MNP) spiked with either Pt or Au at 1, 25, and 100 mg kg⁻¹ using a next-generation sequencing approach (amplicon-based, MiSeq). Soil type and metal concentrations were observed to be key drivers of Pt and Au effects on soil microbial community structure. Different trends were therefore observed in the response of the bacterial community to Pt and Au amendments; however in each soil type, Pt and Au amendment caused a detectable shift in community structure that in most samples was positively correlated with increasing metal concentrations. New dominant groups were only observed in BGR and PPN soils at 100 mg kg⁻¹ (Kazan-3B-28 and Verrucomicrobia groups (BGR, Pt) and Firmicutes and Caldithrix groups (PPN, Pt) and WS2 (BGR, Au). The effects of Pt on soil microbial diversity were largely adverse at 100 mg kg⁻¹ and were pronounced in acidic, basic, and metal/silt-rich soils. However, this effect was concentration-related; Au appeared to be more toxic to soil bacterial communities than Pt at 25 mg kg⁻¹ but Pt was more toxic at 100 mg kg⁻¹. More bacterial groups such as those belonging to Burkholderiales/Burkholderiaceae, Alicyclobacillaceae, Rubrobacteraceae, Cytophagaceae, Oxalobacteraceae were selectively enriched by Pt compared to Au (Sphingomonadaceae and Rhodospirillaceae) amendments irrespective of soil type. The research outcomes have important implications in the management (remediation) of Pt- and Au-contaminated environments.

在四种不同的澳大利亚土壤类型（酸性燃烧场 (BGR)、富含有机质的 Fox Lane、高淤泥/金属 Pinpinio (PPN)、和添加了 1、25 和 100 mg kg ^{-1 的}Pt 或 Au 的碱金属 Minnipa (MNP)使用下一代测序方法（基于扩增子，MiSeq）。观察到土壤类型和金属浓度是 Pt 和 Au 对土壤微生物群落结构影响的关键驱动因素。因此，在细菌群落对 Pt 和 Au 修正物的反应中观察到了不同的趋势；然而，在每种土壤类型中，Pt 和 Au 改良剂导致群落结构发生可检测到的变化，在大多数样品中，这与金属浓度的增加呈正相关。新的优势群仅在 100 mg kg ^{-1 的}BGR 和 PPN 土壤中观察到（Kazan-3B-28和疣微菌群（BGR，Pt）以及厚壁菌群和Caldithrix群（PPN，Pt）和WS2（BGR，金）。Pt 对土壤微生物多样性的影响在 100 mg kg ^{-1 时}主要是不利的，并且在酸性、碱性和富含金属/淤泥的土壤中很明显。然而，这种影响与浓度有关。Au 在 25 mg kg ^{-1 时}似乎比 Pt 对土壤细菌群落的毒性更大，但 Pt 在 100 mg kg ^{-1 时}毒性更大。无论土壤类型如何，与 Au（鞘氨醇单胞菌科和红螺菌科）修正物相比，Pt 选择性地富集了更多的细菌群，例如属于伯克霍尔德氏菌/伯克霍尔德菌科、脂环杆菌科、红杆菌科、噬菌体科、草酸杆菌科的细菌群。研究成果对 Pt 和 Au 污染环境的管理（修复）具有重要意义。