MCM-41 and MCM-48 with niobium were successfully synthesized using 1-tetradecyl-3-methylimidazolium chloride ([C₁₄MI]Cl) as a structure-directing agent. The best Si/Nb molar ratio was chosen (Si/Nb = 20) and the CALB enzyme was immobilized in situ in the synthesized Nb-MCM. SEM micrographs showed the formation of very regular spherical agglomerates with a diameter between 0.25 and 0.75 μm. The material presented a surface area of 954 and 704 m²/g and a pore volume of 0.321 and 0.286 cm³/g, for Nb-MCM-41 and Nb-MCM-48, respectively. Also, both materials showed a pore size of 2.261 nm. The number of recycles obtained for the CALB enzyme immobilized in Nb-MCM-41 and Nb-MCM-48 was 26 recycles with a residual activity of 49.62% and 16 recycles with a residual activity of 53.01%, respectively. For both materials, enzymatic activity remained stable for 5 months of storage at room temperature and refrigeration. The supports were able to catalyze the esterification reaction at 40, 60, and 80 °C, showing industrial application in reactions that require high temperatures. This methodology allows the preparation of new highly active and selective enzyme catalysts using niobium and [C₁₄MI]Cl. Also, the new materials can provide greater viability in processes, ensuring a longer service life of catalysts.

以1-十四烷基-3-甲基咪唑鎓氯化物（[C ₁₄ MI] Cl）为结构导向剂成功合成了含铌的MCM-41和MCM-48 。选择最佳的Si / Nb摩尔比（Si / Nb = 20），并将CALB酶原位固定在合成的Nb-MCM中。SEM显微照片显示形成非常规则的球形附聚物，其直径在0.25至0.75μm之间。该材料的表面积为954和704 m ² / g，孔体积为0.321和0.286 cm ³/ g，分别用于Nb-MCM-41和Nb-MCM-48。而且，两种材料均显示出2.261nm的孔径。固定在Nb-MCM-41和Nb-MCM-48中的CALB酶的回收数分别为26个回收率和49.62％的残留活性，以及​​16个回收率和53.01％的残留活性。对于这两种材料，酶活性在室温和冷藏下保存5个月都保持稳定。载体能够在40、60和80℃下催化酯化反应，在需要高温的反应中显示出工业应用价值。该方法允许使用铌和[C ₁₄ MI] Cl制备新的高活性和选择性酶催化剂。而且，新材料可以在工艺中提供更大的生存能力，从而确保更长的催化剂使用寿命。