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Nb-MCM-Type Mesoporous Material Synthesis Using Ionic Solid as Structure-Directing Agent for In Situ Lipase Immobilization

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Abstract

MCM-41 and MCM-48 with niobium were successfully synthesized using 1-tetradecyl-3-methylimidazolium chloride ([C14MI]Cl) as a structure-directing agent. The best Si/Nb molar ratio was chosen (Si/Nb = 20) and the CALB enzyme was immobilized in situ in the synthesized Nb-MCM. SEM micrographs showed the formation of very regular spherical agglomerates with a diameter between 0.25 and 0.75 μm. The material presented a surface area of 954 and 704 m2/g and a pore volume of 0.321 and 0.286 cm3/g, for Nb-MCM-41 and Nb-MCM-48, respectively. Also, both materials showed a pore size of 2.261 nm. The number of recycles obtained for the CALB enzyme immobilized in Nb-MCM-41 and Nb-MCM-48 was 26 recycles with a residual activity of 49.62% and 16 recycles with a residual activity of 53.01%, respectively. For both materials, enzymatic activity remained stable for 5 months of storage at room temperature and refrigeration. The supports were able to catalyze the esterification reaction at 40, 60, and 80 °C, showing industrial application in reactions that require high temperatures. This methodology allows the preparation of new highly active and selective enzyme catalysts using niobium and [C14MI]Cl. Also, the new materials can provide greater viability in processes, ensuring a longer service life of catalysts.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgments

The authors thank to URI Erechim, National Council for Scientific and Technological Development (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES), and Research Support Foundation of the State of Rio Grande do Sul (FAPERGS).

Funding

The research project was supported by URI Erechim, CNPq, CAPES, and FAPERGS.

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Authors and Affiliations

  1. Department of Food and Chemical Engineering, URI – Erechim, 1621, Sete de Setembro Av., Erechim, RS, 99709-910, Brazil

    Iemedelais Bordin, Victor de Aguiar Pedott, Carolina E. Demaman Oro, Alexander Junges, Rogério Marcos Dallago & Marcelo Luis Mignoni

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  1. Iemedelais Bordin
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  2. Victor de Aguiar Pedott
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  3. Carolina E. Demaman Oro
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  4. Alexander Junges
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  6. Marcelo Luis Mignoni
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Contributions

IB and MM conceptualized the study. All authors analyzed, interpreted the data, and wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Marcelo Luis Mignoni.

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Highlights

• MCM-41 and MCM-48 with niobium and [C14MI]Cl were successfully synthesized

• The best Si/Nb molar ratio chosen was Si/Nb = 20

• CALB immobilized in Nb-MCM-41 and Nb-MCM-48 showed 26 and 16 recycles, respectively

• Enzymatic activity remained stable for 5 months for both supports

• The supports were able to catalyze the esterification reaction at 40, 60, and 80 °C

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Bordin, I., de Aguiar Pedott, V., Demaman Oro, C.E. et al. Nb-MCM-Type Mesoporous Material Synthesis Using Ionic Solid as Structure-Directing Agent for In Situ Lipase Immobilization. Appl Biochem Biotechnol 193, 1072–1085 (2021). https://doi.org/10.1007/s12010-020-03484-7

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  • DOI: https://doi.org/10.1007/s12010-020-03484-7

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