The residue of organochlorine pesticides (OCPs) has been a major pollution problem in our environment. Dichlorodiphenyltrichloroethane (DDT) is one of the most common persistent OCPs that continue to pose a serious risk to human health and the environment. Some treatment methods have been developed to reduce and minimize the adverse impacts of the use of DDT, including biodegradation with brown-rot fungi (BRF). However, DDT degradation using BRF has still low degradation rate and needs a long incubation time. Therefore, the ability of BRF need to be enhanced to degrade DDT. Interaction and effect of bacteria addition on biodegradation of DDT by brown-rot fungus Daedalea dickinsii were investigated. The interaction assay between D. dickinsii with bacteria addition showed that the addition of bacterium Pseudomonas aeruginosa did not provide resistance to the growth of D. dickinsii . Meanwhile, bacterium Bacillus subtilis addition has an inhibitory effect on the growth of D. dickinsii . The addition of 10 ml (1 ml = 1.05 × 10 9 CFU/ml bacteria cell) of P. aeruginosa and B. subtilis was able to improve DDT biodegradation by D. dickinsii from 53.61% to 96.70% and 67.60%, respectively. The highest biodegradation capability of DDT was obtained through addition of 10 ml of P. aeruginosa into the D. dickinsii culture in which the mixed cultures produce final metabolites of 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD) and 1-chloro-2,2-bis(4-chlorophenyl)ethylene (DDMU). This study indicated that the addition of P. aeruginosa can be used for optimization of DDT biodegradation by D. dickinsii .

中文翻译：

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添加细菌对Daedalea dickinsii生物降解二氯二苯三氯乙烷的相互作用和影响

有机氯农药 (OCP) 的残留一直是我们环境中的主要污染问题。二氯二苯基三氯乙烷 (DDT) 是最常见的持久性 OCP 之一，继续对人类健康和环境构成严重威胁。已经开发了一些处理方法来减少和尽量减少使用滴滴涕的不利影响，包括用褐腐真菌 (BRF) 进行生物降解。然而，使用BRF降解DDT的降解率仍然较低，需要较长的孵育时间。因此，需要增强BRF降解DDT的能力。研究了细菌添加对褐腐真菌 Daedalea dickinsii 生物降解 DDT 的相互作用和影响。D. 之间的相互作用分析 添加细菌的 dickinsii 表明添加细菌铜绿假单胞菌并没有对 D. dickinsii 的生长提供抗性。同时，加入枯草芽孢杆菌对D. dickinsii的生长有抑制作用。添加 10 ml（1 ml = 1.05 × 10 9 CFU/ml 细菌细胞）铜绿假单胞菌和枯草芽孢杆菌能够将 D. dickinsii 的 DDT 生物降解率分别从 53.61% 提高到 96.70% 和 67.60%。DDT 的最高生物降解能力是通过将 10 ml 铜绿假单胞菌加入 D. dickinsii 培养物中获得的，其中混合培养物产生 1,1-二氯-2,2-双（4-氯苯基）乙烷的最终代谢物（ DDD) 和 1-氯-2,2-双(4-氯苯基)乙烯 (DDMU)。该研究表明，添加 P.