The composition of microorganisms in the gastrointestinal tract is closely related to the intestinal microenvironments and the exterior growth environments of host. In this study, 16S rDNA sequencing technology was adopted to investigate the influence of fermentation bed on the cecum microorganisms of ducks. Two feeding density treatment groups were set up, including group A (n = 4brids/m2) and group B (n = 6brids/m2). Samples were collected from the intermediate core fermentation layer (10-20 cm) of the fermented mattress materials and from the intestinal contents of ducks at 4, 6 and 8 weeks, respectively. Results showed that Bacteroidetes (20.12-27.17%) and Ruminococcaceae UCG-014 (2.97-10.1%) were the predominant microorganisms in duck cecum, while the Truepera (5.08-6.29%), Pricia (4.44-5.44%) and Luteimonas (3.62-4.99%) were the dominant microorganisms in fermentation mattress material. The cecum bacteria exhibited great difference among different growth periods of the ducks. Increasing the stocking density of ducks had a negative effect on the beneficial bacteria in the cecum. The microbial populations in fermentation mattress material were very different from that in the cecal. In summary, our findings can provide a scientific data for the rational use of fermentation bed feeding mode in poultry production.

中文翻译：

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发酵床对发酵垫料及鸭盲肠细菌生长的影响

胃肠道微生物的组成与肠道微环境和宿主外部生长环境密切相关。本研究采用16S rDNA测序技术研究发酵床对鸭盲肠微生物的影响。设置两个饲养密度处理组，A组（n=4brids/m2）和B组（n=6brids/m2）。分别从发酵垫材料的中间核心发酵层（10-20 cm）和鸭子在第 4、6 和 8 周时的肠道内容物中收集样品。结果表明，拟杆菌（20.12-27.17%）和瘤胃球菌科UCG-014（2.97-10.1%）是鸭盲肠中的主要微生物，而Truepera（5.08-6.29%）、Pricia（4.44-5.44%）和Luteimonas（362）。 -4. 99%) 是发酵床垫材料中的优势微生物。盲肠细菌在鸭子不同生育时期表现出很大差异。增加鸭子的放养密度对盲肠中的有益细菌有负面影响。发酵床垫材料中的微生物种群与盲肠中的微生物种群非常不同。总之，我们的研究结果可以为在家禽生产中合理使用发酵床饲养模式提供科学数据。