A simple new method based on cloud point extraction was optimized and validated to determine spirotetramat in agricultural surface water samples using HPLC–UV. In this method, the nonionic surfactant Triton X-114 was selected as a green extraction solvent because of its structure and properties. The effects of various parameters such as concentration and volume of Triton X-114, pH, temperature, and detergent agent were investigated on spirotetramat recovery and extraction and the data were optimized. Chromatographic separation was performed on the column ZORBAX Eclipse C₁₈ (150 mm × 4.6 mm ID, 5 µm particle size) for the desired composition was detected at 240 nm. Under favorable conditions, the calibration curve was obtained for analyte of linear Dynamic range of 0.5–40 ppm with a correlation coefficient (r²) as 0.9978. In this method, the limit of detection for spirotetramat was 0.08 ppm, and for relative standard deviations (RSD, n = 5) was 4.9%.

优化并验证了一种简单的基于浊点提取的新方法，该方法可使用HPLC-UV测定农业地表水样品中的螺旋藻。在该方法中，由于其结构和性能，非离子表面活性剂Triton X-114被选作绿色萃取溶剂。研究了Triton X-114的浓度和体积，pH，温度和去污剂等各种参数对螺旋四茂铁回收和萃取的影响，并优化了数据。在ZORBAX Eclipse C ₁₈色谱柱（150 mm×4.6 mm ID，5 µm粒径）上进行色谱分离，从而在240 nm处检测到所需的成分。在有利条件下，线性动态范围为0.5–40 ppm的分析物具有相关系数（r ²）为0.9978。在这种方法中，螺旋四溴甲烷的检出限为0.08 ppm，相对标准偏差（RSD，n  = 5）为4.9％。